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PMID: 3614201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.

Molecular and cellular biology ·Vol. 7 ·No. 7 ·1987-07-00 ·Pages 2592-6

Hoffman EK, Trusko SP, Freeman N, George DL

Abstract

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA/genetics Exons Gene Amplification Mice Promoter Regions, Genetic Proto-Oncogenes Transcription, Genetic
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hoffman E K
Trusko S P
Freeman N
George D L
References (37)
37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.7.2592-2596.1987
Published
1987-07-00
Pages
2592-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365395
Subset
IM
Grants
NCI NIH HHS · CA34462 · United States
Databases
GENBANK
M16708
External Links
PubMed source DOI: 10.1128/mcb.7.7.2592-2596.1987 PMC Full Text View journal details
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