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PMID: 3520294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Organ specific expression of ras oncoproteins during growth and development of the rat.

Molecular and cellular biochemistry ·Vol. 70 ·No. 1 ·1986-04-00 ·Pages 97-104

Tanaka T, Ida N, Shimoda H, Waki C, Slamon DJ, Cline MJ

Abstract

Expression of proteins encoded by the ras proto-oncogenes was examined in extracts from normal rat organs using anti-ras p21 antibodies generated against synthetic peptides. The highest level of ras p21 was found in brain (cerebrum) and was predominantly of c-Ha-ras origin. Levels of brain ras p21 did not vary from the newborn period of 3 months of age. Moderate levels of ras p21s were detected in lung, spleen and thymus. In contrast to the p21 in brain, these levels varied with the age of the rats and were encoded by other members of ras proto-oncogene family (Ki-ras or N-ras). This organ specific expression of different ras genes might be related to developmental control of gene expressions.

MeSH Terms
Animals Brain/growth & development Brain Chemistry Gene Expression Regulation Immunoelectrophoresis Oncogenes Organ Specificity Proto-Oncogene Proteins/biosynthesis,genetics,immunology Proto-Oncogene Proteins p21(ras) Rats/growth & development,metabolism Rats, Inbred Strains Sequence Homology, Nucleic Acid
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tanaka T
Ida N
Shimoda H
Waki C
Slamon D J
Cline M J
References (35)
35 references, click to expand
  1. Primary structure of the alpha-subunit of transducin and its relationship to ras proteins.
    Nature. 1985 May 16-22;315 (6016):242-5 PMID: 3923359
  2. Transcription of c-onc genes c-rasKi and c-fms during mouse development.
    Mol Cell Biol. 1983 Jun;3(6):1062-9 PMID: 6308423
  3. Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation.
    Cell. 1984 Feb;36(2):241-7 PMID: 6692471
  4. Effect of divalent metal ions and glycerol on the GTPase activity of H-ras proteins.
    Biochem Biophys Res Commun. 1985 May 16;128(3):1440-6 PMID: 2988539
  5. Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9 PMID: 228288
  6. A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):202-5 PMID: 6582476
  7. Developmental regulation of a Dictyostelium gene encoding a protein homologous to mammalian ras protein.
    Cell. 1984 Nov;39(1):141-8 PMID: 6091907
  8. Expression of cellular oncogenes in human malignancies.
    Science. 1984 Apr 20;224(4646):256-62 PMID: 6538699
  9. Transformation of NIH 3T3 cells by microinjection of Ha-ras p21 protein.
    Nature. 1984 Aug 9-15;310(5977):508-11 PMID: 6611509
  10. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  11. The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activity.
    Nature. 1984 Sep 20-26;311(5983):273-5 PMID: 6148703
  12. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  13. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  14. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.
    Nature. 1981 Aug 6;292(5823):506-11 PMID: 6265801
  15. The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid.
    Cell. 1982 Dec;31(2 Pt 1):465-74 PMID: 6297767
  16. Guanosine nucleotide binding by highly purified Ha-ras-encoded p21 protein produced in Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6953-7 PMID: 6438627
  17. Autophosphorylation of v-Ha-ras p21 is modulated by amino acid residue 12.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2674-8 PMID: 6609366
  18. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  19. Sequence of the alpha subunit of photoreceptor G protein: homologies between transducin, ras, and elongation factors.
    Science. 1985 Apr 5;228(4695):96-9 PMID: 3856323
  20. Differential expression of cellular oncogenes during pre- and postnatal development of the mouse.
    Nature. 1982 Oct 14;299(5884):640-4 PMID: 6289129
  21. Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3400-4 PMID: 3858828
  22. Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1980 Apr;19(4):1005-14 PMID: 6247068
  23. Isolation of transforming sequences of two human lung carcinomas: structural and functional analysis of the activated c-K-ras oncogenes.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):71-5 PMID: 6320174
  24. Mechanism of activation of an N-ras oncogene of SW-1271 human lung carcinoma cells.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3670-4 PMID: 6587382
  25. Comparative biochemical properties of p21 ras molecules coded for by viral and cellular ras genes.
    J Virol. 1982 Nov;44(2):509-19 PMID: 6292515
  26. Expression of cellular oncogenes during embryonic and fetal development of the mouse.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7141-5 PMID: 6594688
  27. G proteins and dual control of adenylate cyclase.
    Cell. 1984 Mar;36(3):577-9 PMID: 6321035
  28. Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells.
    Cell. 1984 Aug;38(1):109-17 PMID: 6380758
  29. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
    Nature. 1985 Jan 17-23;313(5999):241-3 PMID: 3918269
  30. Homologies between signal transducing G proteins and ras gene products.
    Science. 1984 Nov 16;226(4676):860-2 PMID: 6436980
  31. Requirement of either of a pair of ras-related genes of Saccharomyces cerevisiae for spore viability.
    Nature. 1984 Jun 7-13;309(5968):523-7 PMID: 6328319
  32. A brain membrane protein similar to the rat src gene product.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):55-9 PMID: 6264448
  33. DNA sequences homologous to vertebrate oncogenes are conserved in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6789-92 PMID: 6796966
  34. Analysis of the transforming potential of the human H-ras gene by random mutagenesis.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4008-12 PMID: 6330729
  35. Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5704-8 PMID: 6148751
Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00233807
Published
1986-04-00
Pages
97-104
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
NCI NIH HHS · CA15619 · United States
NCI NIH HHS · CA36827 · United States
External Links
PubMed source DOI: 10.1007/BF00233807 View journal details
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