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PMID: 2408272 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 12 ·1985-06-00 ·Pages 3978-82

Farnham PJ, Abrams JM, Schimke RT

Abstract

We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

MeSH Terms
Animals Chromosome Mapping Mice Promoter Regions, Genetic RNA/genetics RNA, Small Nuclear Templates, Genetic Tetrahydrofolate Dehydrogenase/genetics Transcription, Genetic
Chemicals
RNA, Small Nuclear RNA Tetrahydrofolate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Farnham P J
Abrams J M
Schimke R T
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.12.3978
Published
1985-06-00
Pages
3978-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397917
Subset
IM
Grants
NCI NIH HHS · CA 16318 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.12.3978 PMC Full Text View journal details
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