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PMID: 3018531 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.

Molecular and cellular biology ·Vol. 5 ·No. 8 ·1985-08-00 ·Pages 1847-58

Crouse GF, Leys EJ, McEwan RN, Frayne EG, Kellems RE

Abstract

The use of murine dihydrofolate reductase (dhfr) gene amplification mutants enabled us to identify important structural and functional features of the dhfr promoter region. We found another transcription unit, at least 14 kilobases in size, which initiates within 130 base pairs of the major dhfr transcript and is transcribed divergently. The 5' ends of both transcripts were analyzed and found to have multiple initiation sites. The major dhfr transcript and the divergent transcript appear to share the same promoter region; the longer transcripts of the dhfr gene overlap with the divergent transcripts and use a different promoter region. The divergent transcript appears to code for a protein; an homologous sequence to its first exon is found in the corresponding location near the human dhfr gene.

MeSH Terms
Amanitins/pharmacology Animals Base Sequence Cloning, Molecular Dose-Response Relationship, Radiation Endonucleases Gene Amplification Genes/drug effects Mice Mutation Peptide Chain Initiation, Translational Promoter Regions, Genetic Single-Strand Specific DNA and RNA Endonucleases Tetrahydrofolate Dehydrogenase/genetics Transcription, Genetic/drug effects,radiation effects Ultraviolet Rays
Chemicals
Amanitins Tetrahydrofolate Dehydrogenase Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Crouse G F
Leys E J
McEwan R N
Frayne E G
Kellems R E
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35 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.8.1847-1858.1985
Published
1985-08-00
Pages
1847-58
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366900
Subset
IM
Grants
NCI NIH HHS · 1 KO4 CA00828 · United States
NIGMS NIH HHS · GM 30204 · United States
NCI NIH HHS · N01-CO-23909 · United States
Databases
GENBANK
M11419
External Links
PubMed source DOI: 10.1128/mcb.5.8.1847-1858.1985 PMC Full Text View journal details
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