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PMID: 6548307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues.

Nucleic acids research ·Vol. 12 ·No. 18 ·1984-09-25 ·Pages 6951-63

Piechaczyk M, Blanchard JM, Marty L, Dani C, Panabieres F, El Sabouty S, Fort P, Jeanteur P

Abstract

We have isolated and identified cDNA clones containing part of the coding sequence for rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C. 1.2.1.12). By using one of these clones as a probe, we have shown that: i) the abundance of GAPDH mRNA is different in various tissues of the adult rat and in good correlation with the abundance of the enzyme; ii) the transcription rates are quite similar in all tissues tested. We therefore conclude that the tissue-specific differential GAPDH gene expression is regulated by adjusting the abundance of its mRNA at the post-transcriptional level.

MeSH Terms
Animals Base Sequence Cell Line Cell Nucleus/metabolism Cloning, Molecular DNA/isolation & purification Gene Expression Regulation Genes Glioma/enzymology Glyceraldehyde-3-Phosphate Dehydrogenases/genetics Male Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Rats Tissue Distribution Transcription, Genetic
Chemicals
RNA, Messenger DNA Glyceraldehyde-3-Phosphate Dehydrogenases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Piechaczyk M
Blanchard J M
Marty L
Dani C
Panabieres F
El Sabouty S
Fort P
Jeanteur P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.18.6951
Published
1984-09-25
Pages
6951-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320135
Subset
IM
Databases
GENBANK
X00972
External Links
PubMed source DOI: 10.1093/nar/12.18.6951 PMC Full Text View journal details
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