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PMID: 2535736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of origin of DNA replication of human papovavirus BK.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 356-65

Deyerle KL, Sajjadi FG, Subramani S

Abstract

We have analyzed the cis-acting sequence elements and properties of the origin of DNA replication of human papovavirus BK (BKV). The precise boundaries of the origin varied, depending on the cell type and the viral T antigen used for assay. The BKV minimal origin of replication consisted of an inverted repeat, T-antigen-binding site II, and a 20-base-pair AT block when assayed in monkey kidney CV1 and HeLa cells by using the BKV T antigen. This 76-base-pair minimal origin did not replicate in COS cells in the presence of the simian virus 40 (SV40) T antigen. Unlike that from the SV40 minimal origin, replication from the BKV minimal origin was not enhanced by BKV ori-flanking sequences in CV1 or HeLa cells, using the BKV T antigen. BKV ori-flanking sequences did activate the SV40 minimal origin of replication in COS cells and relieved the orientation-dependent property of this origin. Finally, the BKV T antigen was found to autoregulate activity of the BKV early transcriptional regulatory region. The BKV origin of replication shows similarities to and differences from those of the related viruses SV40 and polyomavirus, suggesting that the proteins involved in the initiation of replication interact with origin sequences differently in these viruses.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics BK Virus/genetics,physiology Cell Line Cell Line, Transformed DNA Replication HeLa Cells Humans Mutation Plasmids Polyomavirus/genetics Virus Replication
Chemicals
Antigens, Polyomavirus Transforming
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deyerle K L
Department of Biology, University of California, San Diego, La Jolla 92093.
Sajjadi F G
Subramani S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.356-365.1989
Published
1989-01-00
Pages
356-65
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247691
Subset
IM
Grants
NCI NIH HHS · CA44997 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.1.356-365.1989 PMC Full Text View journal details
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