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PMID: 6086955 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Essential nucleotides in the polyomavirus origin region.

Journal of virology ·Vol. 51 ·No. 2 ·1984-08-00 ·Pages 437-44

Triezenberg SJ, Folk WR

Abstract

We have isolated a series of point mutants in the polyomavirus origin-intergenic control region by using a procedure which exploits the single-stranded nature of DNAs cloned into M13 phage both for the generation of mutants and for their analysis by DNA sequencing. In this report we describe the effects of cytosine X guanine----thymine X adenine base-pair substitutions in the polyomavirus origin region upon replication in mouse 3T6 cells of the M13-polyomavirus constructs. Our results indicate that sequences near the center of a 34-base-pair sequence with dyad symmetry are important for replication, whereas specific nucleotides near the ends of the dyad symmetry are not important. Furthermore, a putative large T antigen-binding site at nucleotides 60 to 80 can be mutated without affecting replication function as measured in this assay.

MeSH Terms
Animals Base Composition Base Sequence Cells, Cultured Coliphages/genetics DNA/analysis DNA Restriction Enzymes Escherichia coli/genetics Genes, Viral Genetic Vectors Mice Mutation Polyomavirus/genetics Xenopus
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Triezenberg S J
Folk W R
References (37)
37 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.51.2.437-444.1984
Published
1984-08-00
Pages
437-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254457
Subset
IM
Grants
NCI NIH HHS · CA 13978 · United States
NIGMS NIH HHS · GM 07315 · United States
Databases
GENBANK
J02288, J02289, J02290, J02291, J02292, K00932, K00997, K01041, K01071, K01072, V01117, V01147, V01151
External Links
PubMed source DOI: 10.1128/JVI.51.2.437-444.1984 PMC Full Text View journal details
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