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PMID: 6086311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.

The EMBO journal ·Vol. 3 ·No. 6 ·1984-06-00 ·Pages 1243-53

Cereghini S, Yaniv M

Abstract

Chimeric SV40 DNA containing only the early region, or plasmid DNA harboring the origin-promoter-enhancer region of SV40, when introduced into CV-1 or Cos-1 monkey cells by DEAE-dextran mediated transfer are rapidly assembled in a typical chromatin structure revealed by the generation of a regular 190 bp repeat ladder after micrococcal nuclease digestion. DNA replication is not required for this assembly process. Chromatin-specific DNase I hypersensitive sites are observed in the enhancer region of these minichromosomes. The pattern of the sites differs between non-replicating and post-replicated chromatin. The latter is identical to that observed in the lytic cycle. The presence of large T antigen is not sufficient for the shift in the structure of the chromatin. These experiments suggest that replication can modulate protein-DNA interactions during viral infection or upon cell differentiation.

MeSH Terms
Animals Base Sequence Cell Line Chlorocebus aethiops Chromatin/physiology DNA Replication DNA Restriction Enzymes DNA, Recombinant/analysis DNA, Viral/genetics Kidney Operon Plasmids Simian virus 40/genetics Transfection
Chemicals
Chromatin DNA, Recombinant DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cereghini S
Yaniv M
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65 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-06-00
Pages
1243-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557505
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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