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PMID: 2526337 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mouse Mos protooncogene product is present and functions during oogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5395-9

Paules RS, Buccione R, Moschel RC, Vande Woude GF, Eppig JJ

Abstract

We have identified the mouse Mos-encoded protein product, p39mos, in maturing mouse oocytes and have shown that it is indistinguishable from the product expressed in Mos-transformed NIH 3T3 cells. p39mos is detected in oocytes arrested in the first meiotic prophase, during germinal-vesicle breakdown, metaphase I, anaphase I, and in ovulated eggs. We show that microinjection of three different Mos antisense (but not sense) oligodeoxyribonucleotides into germinal vesicle-stage oocytes prevents first polar-body emission and therefore interrupted the normal progression of meiosis. These results show that in mouse oocytes, as in the amphibian Xenopus [Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. & Vande Woude, G.F. (1988) Nature (London) 335, 519-525], the product of Mos is necessary for normal meiotic maturation.

MeSH Terms
Animals Base Sequence Cells, Cultured Female Meiosis Mice Molecular Sequence Data Oligonucleotide Probes Oocytes/cytology,physiology Oogenesis Proto-Oncogene Proteins/genetics,physiology Proto-Oncogene Proteins c-mos Proto-Oncogenes Xenopus
Chemicals
Oligonucleotide Probes Proto-Oncogene Proteins Proto-Oncogene Proteins c-mos
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Paules R S
BRI-Basic Research Program, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
Buccione R
Moschel R C
Vande Woude G F
Eppig J J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5395
Published
1989-07-00
Pages
5395-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297629
Subset
IM
Grants
NICHD NIH HHS · HD20575 · United States
NCI NIH HHS · N01-CO-74101 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.14.5395 PMC Full Text View journal details
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