Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

Nucleic acids research ·Vol. 14 ·No. 13 ·1986-07-11 ·Pages 5399-407

Froehler BC, Ng PG, Matteucci MD

Abstract

Deoxynucleoside H-phosphonates are used in the chemical synthesis of deoxyoligonucleotides up to 107 bases in length. The biological activity of the synthetic DNA is assessed by cloning into M13 and sequencing. An improved synthesis of protected deoxynucleoside H-phosphonates is also described.

MeSH Terms

Chemical Phenomena Chemistry DNA/chemical synthesis Deoxyribonucleosides Molecular Weight Organophosphonates

Chemicals

Deoxyribonucleosides Organophosphonates DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Froehler B C

Ng P G

Matteucci M D

References (4)

4 references, click to expand

DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.
Nucleic Acids Res. 1985 Sep 25;13(18):6447-65 PMID: 4059050
Dialkylformamidines: depurination resistant N6-protecting group for deoxyadenosine.
Nucleic Acids Res. 1983 Nov 25;11(22):8031-6 PMID: 6606157
Syringe method for stepwise chemical synthesis of oligonucleotides.
Nucleic Acids Res. 1982 May 25;10(10):3249-60 PMID: 7099961

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.13.5399

Published

1986-07-11

Pages

5399-407

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311548

Subset

External Links

PubMed source DOI: 10.1093/nar/14.13.5399 PMC Full Text View journal details

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Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

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