Home LiteratureArticle Details
PMID: 2825169 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 22 ·1987-11-00 ·Pages 7896-900

Dash P, Lotan I, Knapp M, Kandel ER, Goelet P

Abstract

Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.

MeSH Terms
Animals Base Sequence Calmodulin/genetics Cell-Free System Endoribonucleases/metabolism Female Gene Expression Regulation Globins/genetics Oligodeoxyribonucleotides/chemical synthesis,pharmacology Oocytes/metabolism Protein Biosynthesis RNA, Messenger/metabolism Rabbits Ribonuclease H Triticum Xenopus laevis
Chemicals
Calmodulin Oligodeoxyribonucleotides RNA, Messenger Globins Endoribonucleases Ribonuclease H
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dash P
Howard Hughes Medical Institute, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY.
Lotan I
Knapp M
Kandel E R
Goelet P
References (26)
26 references, click to expand
  1. Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.
    Cell. 1985 Aug;42(1):129-38 PMID: 2410135
  2. Transcription unit of the rabbit beta 1 globin gene.
    Mol Cell Biol. 1985 Jan;5(1):147-60 PMID: 2580228
  3. Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
    Nature. 1985 Feb 21-27;313(6004):703-6 PMID: 2579337
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert.
    J Biol Chem. 1986 Jan 15;261(2):839-48 PMID: 3001084
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  8. Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4143-6 PMID: 3012555
  9. The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.
    Nucleic Acids Res. 1986 Aug 26;14(16):6433-51 PMID: 3018671
  10. Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
    Cell. 1987 Feb 27;48(4):599-605 PMID: 2434240
  11. Inhibition of gene expression in plant cells by expression of antisense RNA.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5372-6 PMID: 16593734
  12. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
  13. A developmentally regulated activity that unwinds RNA duplexes.
    Cell. 1987 Feb 27;48(4):607-13 PMID: 2434241
  14. Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes.
    Nucleic Acids Res. 1985 Jul 11;13(13):4991-5004 PMID: 3875079
  15. Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
    J Cell Biol. 1985 Sep;101(3):1094-9 PMID: 2411734
  16. Isolation and characterization of calmodulin genes from Xenopus laevis.
    Mol Cell Biol. 1984 Mar;4(3):507-13 PMID: 6325880
  17. Constitutive and conditional suppression of exogenous and endogenous genes by anti-sense RNA.
    Science. 1985 Jul 26;229(4711):345-52 PMID: 2990048
  18. Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.
    Cell. 1984 Apr;36(4):1007-15 PMID: 6323013
  19. Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):144-8 PMID: 3855537
  20. Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.
    Nucleic Acids Res. 1987 Jun 25;15(12):4717-36 PMID: 3037483
  21. Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2787-91 PMID: 3010316
  22. Enzyme from calf thymus degrading the RNA moiety of DNA-RNA Hybrids: effect on DNA-dependent RNA polymerase.
    Science. 1969 Oct 17;166(3903):393-5 PMID: 5812039
  23. Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
    Science. 1986 Mar 7;231(4742):1147-50 PMID: 2418503
  24. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370-4 PMID: 270678
  25. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
    Nature. 1971 Sep 17;233(5316):177-82 PMID: 4939175
  26. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.22.7896
Published
1987-11-00
Pages
7896-900
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299442
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.22.7896 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2