Specific inhibition of endogenous beta-tubulin synthesis in Xenopus oocytes by anti-messenger oligodeoxynucleotides.

Nucleic acids research ·Vol. 16 ·No. 5 ·1988-03-25 ·Pages 2225-33

Jessus C, Cazenave C, Ozon R, Hélène C

Abstract

An oligodeoxynucleotide containing 27 nucleotides, complementary to a highly conserved sequence of beta-tubulin mRNAs, led to a nearly complete inhibition of beta-tubulin synthesis in Xenopus oocytes after microinjection. Inhibition persisted 24 hours post-injection and was specific for beta-tubulin as the synthesis of alpha-tubulin as well as that of other proteins from the oocyte was not affected. Complete inhibition of beta-tubulin synthesis did not prevent progesterone-induced meiotic maturation and formation of the chromosome spindle. This result indicates that the pool of endogenous tubulin already present in fully-grown oocytes is sufficient to allow normal meiotic maturation. This finding correlates with previous experiments showing that the turn-over of tubulin is very slow in the oocyte.

MeSH Terms

Animals Gene Expression Regulation/drug effects Meiosis Microinjections Oligodeoxyribonucleotides/pharmacology Oocytes/physiology RNA, Messenger/genetics Tubulin/biosynthesis,genetics Xenopus laevis

Chemicals

Oligodeoxyribonucleotides RNA, Messenger Tubulin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jessus C

Laboratoire de Physiologie de la Reproduction, INRA CNRS UA 555, Université Pierre et Marie Curie, Paris, France.

Cazenave C

Ozon R

Hélène C

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.5.2225

Published

1988-03-25

Pages

2225-33

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338211

Subset

External Links

PubMed source DOI: 10.1093/nar/16.5.2225 PMC Full Text View journal details

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Specific inhibition of endogenous beta-tubulin synthesis in Xenopus oocytes by anti-messenger oligodeoxynucleotides.

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