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PMID: 2832744 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chicken homolog of the mos proto-oncogene.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 923-9

Schmidt M, Oskarsson MK, Dunn JK, Blair DG, Hughes S, Propst F, Vande Woude GF

Abstract

We compared the sequence and properties of the chicken mos homolog with the previously characterized mouse and human c-mos genes. Sequence analysis revealed one major open reading frame of 1,047 base pairs encoding a protein of 349 amino acids. Both the nucleotide sequence and the deduced amino acid sequence showed 62% overall homology to mouse and human c-mos, but regions of higher conservation (approximately 70%) occurred in the putative ATP-binding and kinase domains. We detected mos transcripts by Northern (RNA) analyses in RNA prepared from chicken and quail ovaries and testes. Evidence for low levels of mos RNA expression in adult chicken heart, kidney, and spleen and in the entire embryo was obtained by S1 nuclease protection experiments. In contrast to the low transforming efficiency of human c-mos when linked to a mouse retroviral long terminal repeat element, chicken c-mos transformed NIH 3T3 cells as efficiently as mouse c-mos did. We also show that chicken primary embryo fibroblasts were morphologically altered when infected with an avian retroviral vector containing the chicken c-mos coding region.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Chickens/genetics Cloning, Molecular Codon DNA Restriction Enzymes Female Humans Male Mice Molecular Sequence Data Proto-Oncogene Mas Proto-Oncogene Proteins/genetics Proto-Oncogenes Sequence Homology, Nucleic Acid
Chemicals
Codon MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schmidt M
Bionetics Research, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
Oskarsson M K
Dunn J K
Blair D G
Hughes S
Propst F
Vande Woude G F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.923-929.1988
Published
1988-02-00
Pages
923-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363224
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
M19412
External Links
PubMed source DOI: 10.1128/mcb.8.2.923-929.1988 PMC Full Text View journal details
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