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PMID: 2457111 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.

Journal of virology ·Vol. 62 ·No. 9 ·1988-09-00 ·Pages 3109-19

O'Reilly DR, Miller LK

Abstract

Recombinant baculoviruses were constructed which express simian virus 40 large T antigen (SVT-Ag) or murine p53 to high levels in infected insect cells. Characterization of the expressed proteins revealed that they display many properties of the corresponding mammalian-derived proteins. Both proteins are of wild-type size, localize to the nucleus, are recognized by several SVT-Ag- or p53-specific monoclonal antibodies, and are phosphorylated in this system. Complexes are formed between baculovirus-derived SVT-Ag and p53 after coinfection of insect cells with both recombinant viruses. After infection of insect cells with either virus individually, each protein can self-associate to form a variety of oligomeric species. Pulse-chase experiments indicated that both SVT-Ag and p53 are highly stable in insect cells, even in the absence of complex formation.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/analysis,biosynthesis,genetics,metabolism Cell Line Cell Nucleus/metabolism Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Epitopes/analysis Fluorescent Antibody Technique Gene Expression Regulation Genetic Vectors Immunoassay Insect Viruses/genetics Kinetics Mice Moths Neoplasm Proteins/analysis,biosynthesis,genetics,metabolism Nuclear Proteins/analysis,biosynthesis,genetics,metabolism Phosphoproteins/analysis,biosynthesis,genetics,metabolism Phosphorylation Plasmids Simian virus 40/genetics,immunology Tumor Suppressor Protein p53
Chemicals
Antigens, Polyomavirus Transforming Epitopes Neoplasm Proteins Nuclear Proteins Phosphoproteins Tumor Suppressor Protein p53
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Reilly D R
Department of Entomology, University of Georgia, Athens 30602.
Miller L K
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55 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.9.3109-3119.1988
Published
1988-09-00
Pages
3109-19
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253427
Subset
IM
Grants
NIAID NIH HHS · AI23719 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.9.3109-3119.1988 PMC Full Text View journal details
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