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PMID: 3033300 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of polyomavirus large T antigen by using a baculovirus vector.

Journal of virology ·Vol. 61 ·No. 5 ·1987-05-00 ·Pages 1712-6

Rice WC, Lorimer HE, Prives C, Miller LK

Abstract

A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Viral, Tumor/genetics Cloning, Molecular DNA-Binding Proteins/genetics Genetic Vectors Insect Viruses/genetics Occlusion Body Matrix Proteins Polyomavirus/genetics Promoter Regions, Genetic Viral Proteins/genetics Viral Structural Proteins
Chemicals
Antibodies, Monoclonal Antigens, Viral, Tumor DNA-Binding Proteins Occlusion Body Matrix Proteins Viral Proteins Viral Structural Proteins polyhedrin protein, Nucleopolyhedrovirus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rice W C
Lorimer H E
Prives C
Miller L K
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.5.1712-1716.1987
Published
1987-05-00
Pages
1712-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254162
Subset
IM
Grants
NIAID NIH HHS · AI 23719 · United States
NCI NIH HHS · CA26905 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.5.1712-1716.1987 PMC Full Text View journal details
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