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PMID: 2983341 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An adenovirus vector system used to express polyoma virus tumor antigens.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 5 ·1985-03-00 ·Pages 1359-63

Mansour SL, Grodzicker T, Tjian R

Abstract

We have used a generalized adenovirus vector system to express the three polyoma tumor (T) antigen proteins under the control of the adenovirus major late promoter. One hybrid virus, Ad-PySVR498, expresses high levels of polyoma middle and small T antigens. A second hybrid virus, Ad-LTSVR545, which contains a cDNA copy of the polyoma A gene, overproduces large T antigen. The T antigens produced are indistinguishable from their authentic polyoma counterparts as determined by immunoprecipitation and partial cleavage by V8 protease. Analysis of polyoma mRNAs encoded by the recombinant viruses showed that they initiate from the adenovirus major late promoter and contain the tripartite leader at their 5' ends. Large T antigen isolated from Ad-LTSVR545-infected cells by immunoaffinity was shown to bind selectively to polyoma DNA sequences that contain the origin of viral DNA replication as well as the sites for transcription initiation.

MeSH Terms
Adenoviridae/genetics Antigens, Viral, Tumor/genetics DNA, Recombinant Gene Expression Regulation Genes, Viral Genetic Vectors Polyomavirus/genetics RNA, Messenger/genetics
Chemicals
Antigens, Viral, Tumor DNA, Recombinant RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mansour S L
Grodzicker T
Tjian R
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.5.1359
Published
1985-03-00
Pages
1359-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397260
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.5.1359 PMC Full Text View journal details
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