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PMID: 6095261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of polyomavirus transcription by large tumor antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 22 ·1984-11-00 ·Pages 6919-23

Farmerie WG, Folk WR

Abstract

We have analyzed the regulation of viral transcription by the large tumor antigen in cells infected by several viable deletion and insertion mutants of polyomavirus. We find that deletion of the early promoter "TATA box" and associated large tumor antigen binding site has only a small effect on the balance of early and late mRNAs. Furthermore, transcription of a polyomavirus containing a heterologous adenovirus promoter in place of the normal TATA box and cap sites is regulated by the large tumor antigen. We conclude that repression of polyomavirus early transcription cannot occur simply by binding of the large tumor antigen to DNA sequences at the site of transcription initiation and must involve the interaction of the large tumor antigen binding at other sites.

MeSH Terms
Antigens, Viral, Tumor/genetics Base Sequence Gene Expression Regulation Polyomavirus/genetics Promoter Regions, Genetic RNA Polymerase II/genetics RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Transcription, Genetic
Chemicals
Antigens, Viral, Tumor RNA, Messenger RNA, Viral RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Farmerie W G
Folk W R
References (46)
46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.22.6919
Published
1984-11-00
Pages
6919-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392047
Subset
IM
Grants
NCI NIH HHS · 5T32 CA09281 · United States
NCI NIH HHS · CA13978 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.22.6919 PMC Full Text View journal details
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