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PMID: 3878519 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8404-8

Smith GE, Ju G, Ericson BL, Moschera J, Lahm HW, Chizzonite R, Summers MD

Abstract

A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.

MeSH Terms

Amino Acid Sequence Animals Gene Expression Regulation Genetic Vectors Glycoproteins/genetics,metabolism Humans Insect Viruses/genetics Insecta/genetics Interleukin-2/biosynthesis,genetics,metabolism Molecular Weight Protein Processing, Post-Translational Recombinant Proteins/biosynthesis,genetics,metabolism

Chemicals

Glycoproteins Interleukin-2 Recombinant Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Smith G E

Ju G

Ericson B L

Moschera J

Lahm H W

Chizzonite R

Summers M D

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.24.8404

Published

1985-12-00

Pages

8404-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC390924

Subset

External Links

PubMed source DOI: 10.1073/pnas.82.24.8404 PMC Full Text View journal details

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Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

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