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PMID: 6604277 Published · ppublish English Journal Article

Purification and partial sequence analysis of human T-cell growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 19 ·1983-10-00 ·Pages 5990-4

Robb RJ, Kutny RM, Chowdhry V

Abstract

A murine monoclonal antibody directed against human T-cell growth factor (TCGF) from the JURKAT cell line was used for affinity column purification of the factor. Bound TCGF was eluted nearly quantitatively at low pH, and the recovered factor appeared homogeneous by two-dimensional gel electrophoresis. The molecule is markedly hydrophobic, with a high content of leucine. A single NH2-terminal sequence of 36 residues was obtained by automated Edman degradation, further supporting the homogeneity of the material. Thus, significant quantities of purified TCGF have been prepared in a single step, making possible detailed analysis of its molecular structure and biological role.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Cell Division Cell Line Chromatography, Affinity/methods Humans Interleukin-2/isolation & purification Leukemia/immunology,physiopathology T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Interleukin-2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Robb R J
Kutny R M
Chowdhry V
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.19.5990
Published
1983-10-00
Pages
5990-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC534345
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.19.5990 PMC Full Text View journal details
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