Home LiteratureArticle Details
PMID: 3027397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Abundant synthesis of functional human T-cell leukemia virus type I p40x protein in eucaryotic cells by using a baculovirus expression vector.

Journal of virology ·Vol. 61 ·No. 3 ·1987-03-00 ·Pages 708-13

Jeang KT, Giam CZ, Nerenberg M, Khoury G

Abstract

The human T-cell leukemia virus type I (HTLV-I) p40x protein is a 40-kilodalton polypeptide encoded in the 3'-terminal region of the virus. This protein is responsible for positive transcriptional trans-activation of promoter elements located within the HTLV-I long terminal repeat. We introduced the protein-coding region of HTLV-I p40x into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus. After infection of the insect Spodoptera frugiperda (SF9) cell line, this recombinant strain of baculovirus produced approximately 200 mg of intact p40x protein per 2.5 X 10(8) cells. The protein was biologically active in trans-activation of an HTLV-I long terminal repeat-human beta-globin construct. Biochemical analyses of the protein suggest that the p40x polypeptide underwent posttranslational modification in these eucaryotic SF9 cells.

MeSH Terms
Biological Assay Cell Line Cloning, Molecular Deltaretrovirus/genetics Gene Expression Regulation Genetic Vectors Insect Viruses/genetics Insecta Isoelectric Point Molecular Weight Protein Processing, Post-Translational Transcription, Genetic Viral Proteins/genetics
Chemicals
Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jeang K T
Giam C Z
Nerenberg M
Khoury G
References (35)
35 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  3. Purification of biologically active simian virus 40 small tumor antigen.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):906-10 PMID: 6302673
  4. E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
    Mol Cell Biol. 1985 Oct;5(10):2653-61 PMID: 2942760
  5. Trans-acting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells.
    Science. 1984 Jul 27;225(4660):381-5 PMID: 6330891
  6. The x gene is essential for HTLV replication.
    Science. 1985 Jul 5;229(4708):54-8 PMID: 2990037
  7. Direct evidence that p40x of human T-cell leukemia virus type I is a trans-acting transcriptional activator.
    EMBO J. 1986 Mar;5(3):561-5 PMID: 3011413
  8. The pX protein of HTLV-I is a transcriptional activator of its long terminal repeats.
    Science. 1985 Aug 16;229(4714):675-9 PMID: 2992082
  9. HTLV x-gene product: requirement for the env methionine initiation codon.
    Science. 1985 Jun 28;228(4707):1534-7 PMID: 2990032
  10. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031-5 PMID: 6979048
  11. Long terminal repeat structure of an American isolate of type I human T-cell leukemia virus.
    Virology. 1984 Dec;139(2):340-5 PMID: 6097028
  12. Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA.
    J Virol. 1980 Jan;33(1):167-82 PMID: 6245226
  13. Natural antibodies to the structural core protein (p24) of the human T-cell leukemia (lymphoma) retrovirus found in sera of leukemia patients in Japan.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1653-7 PMID: 6951204
  14. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  15. A cycloheximide-enhanced protein in cytomegalovirus-infected cells.
    Virology. 1980 Dec;107(2):362-74 PMID: 6256937
  16. p27x-III and p21x-III, proteins encoded by the pX sequence of human T-cell leukemia virus type I.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8359-63 PMID: 3001699
  17. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  18. Functions of purified E1A protein microinjected into mammalian cells.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6988-92 PMID: 6334304
  19. Production of human beta interferon in insect cells infected with a baculovirus expression vector.
    Mol Cell Biol. 1983 Dec;3(12):2156-65 PMID: 6318086
  20. Transcriptional "silencer" element in rat repetitive sequences associated with the rat insulin 1 gene locus.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3151-5 PMID: 3010279
  21. Molecular Engineering of the Autographa californica Nuclear Polyhedrosis Virus Genome: Deletion Mutations Within the Polyhedrin Gene.
    J Virol. 1983 May;46(2):584-93 PMID: 16789242
  22. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8404-8 PMID: 3878519
  23. A transcriptional enhancer sequence of HTLV-I is responsible for trans-activation mediated by p40 chi HTLV-I.
    EMBO J. 1986 Apr;5(4):713-8 PMID: 3011423
  24. Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476-80 PMID: 7031654
  25. Transformation of rat cells by DNA of human adenovirus 5.
    Virology. 1973 Aug;54(2):536-9 PMID: 4737663
  26. Expression of the complete human T-cell leukemia virus type I pX coding sequence as a functional protein in Escherichia coli.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7192-6 PMID: 3020538
  27. Identification and characterization of multiple forms of actin.
    Cell. 1976 Dec;9(4 PT 2):793-805 PMID: 1017015
  28. DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state.
    J Virol. 1984 Apr;50(1):1-12 PMID: 6321781
  29. Expression in Escherichia coli of chemically synthesized genes for human insulin.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):106-10 PMID: 85300
  30. Production of human c-myc protein in insect cells infected with a baculovirus expression vector.
    Mol Cell Biol. 1985 Oct;5(10):2860-5 PMID: 3915537
  31. Identification of the putative transforming protein of the human T-cell leukemia viruses HTLV-I and HTLV-II.
    Science. 1984 Oct 5;226(4670):61-5 PMID: 6089351
  32. Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional.
    Science. 1984 Jun 22;224(4655):1343-6 PMID: 6374895
  33. Expression of the pX gene of HTLV-I: general splicing mechanism in the HTLV family.
    Science. 1985 Jun 28;228(4707):1532-4 PMID: 2990031
  34. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  35. Association of the pX gene product of human T-cell leukemia virus type-I with nucleus.
    Virology. 1985 Dec;147(2):462-5 PMID: 3000074
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.3.708-713.1987
Published
1987-03-00
Pages
708-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254010
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.3.708-713.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2