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PMID: 24466316 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.

PloS one ·Vol. 9 ·No. 1 ·2014-00-00 ·Pages e87016

Kim S, Rhee K

Abstract

At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-protein interaction properties of CEP215 to its biological functions. The results showed that CEP215 and pericentrin, another major PCM component, is interdependent for their accumulation at the spindle poles during mitosis. As a result, The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. On the other hand, CEP215 interaction with γ-tubulin is dispensable for centrosome maturation. Our results provide an insight how PCM components are assembled to form a spindle pole during mitosis.

MeSH Terms
Antigens/chemistry,metabolism Cell Cycle Proteins Centrosome/metabolism,physiology HEK293 Cells HeLa Cells Humans Immunoblotting Immunohistochemistry Immunoprecipitation Intracellular Signaling Peptides and Proteins/metabolism Microscopy, Fluorescence Mitosis/physiology Models, Molecular Nerve Tissue Proteins/metabolism RNA, Small Interfering/genetics Spindle Apparatus/genetics,physiology Tubulin
Chemicals
Antigens CDK5RAP2 protein, human Cell Cycle Proteins Intracellular Signaling Peptides and Proteins Nerve Tissue Proteins RNA, Small Interfering Tubulin pericentrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kim Seongjae
Department of Biological Sciences, Seoul National University, Seoul, Korea.
Rhee Kunsoo
Department of Biological Sciences, Seoul National University, Seoul, Korea.
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34 references, click to expand
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0087016
Published
2014-00-00
Epub
2014-00-22
Pages
e87016
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3899370
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0087016 PMC Full Text View journal details
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