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PMID: 16461362 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly.

The Journal of cell biology ·Vol. 172 ·No. 4 ·2006-02-13 ·Pages 505-15

Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A

Abstract

The centrosome is the major microtubule organizing structure in somatic cells. Centrosomal microtubule nucleation depends on the protein gamma-tubulin. In mammals, gamma-tubulin associates with additional proteins into a large complex, the gamma-tubulin ring complex (gammaTuRC). We characterize NEDD1, a centrosomal protein that associates with gammaTuRCs. We show that the majority of gammaTuRCs assemble even after NEDD1 depletion but require NEDD1 for centrosomal targeting. In contrast, NEDD1 can target to the centrosome in the absence of gamma-tubulin. NEDD1-depleted cells show defects in centrosomal microtubule nucleation and form aberrant mitotic spindles with poorly separated poles. Similar spindle defects are obtained by overexpression of a fusion protein of GFP tagged to the carboxy-terminal half of NEDD1, which mediates binding to gammaTuRCs. Further, we show that depletion of NEDD1 inhibits centriole duplication, as does depletion of gamma-tubulin. Our data suggest that centriole duplication requires NEDD1-dependent recruitment of gamma-tubulin to the centrosome.

MeSH Terms
Cell Line Cell Line, Tumor Cells, Cultured Centrioles/metabolism Centrosome/metabolism Cloning, Molecular DNA, Complementary/genetics Drosophila Proteins/genetics Escherichia coli/genetics HeLa Cells Humans Microtubule-Associated Proteins/genetics,metabolism Protein Binding Spindle Apparatus/metabolism Tubulin/metabolism
Chemicals
DNA, Complementary Drosophila Proteins Grip71 protein, Drosophila Microtubule-Associated Proteins NEDD1 protein, human Tubulin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Haren Laurence
Institut de Sciences et Technologies du Médicament de Toulouse, Centre National de la Recherche Scientifique/Pierre Fabre, 31400 Toulouse, France.
Remy Marie-Hélène
Bazin Ingrid
Callebaut Isabelle
Wright Michel
Merdes Andreas
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200510028
Published
2006-02-13
Epub
2006-00-06
Pages
505-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063671
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200510028 PMC Full Text View journal details
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