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PMID: 17959831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex.

Molecular biology of the cell ·Vol. 19 ·No. 1 ·2008-01-00 ·Pages 115-25

Fong KW, Choi YK, Rattner JB, Qi RZ

Abstract

Microtubule nucleation and organization by the centrosome require gamma-tubulin, a protein that exists in a macromolecular complex called the gamma-tubulin ring complex (gammaTuRC). We report characterization of CDK5RAP2, a novel centrosomal protein whose mutations have been linked to autosomal recessive primary microcephaly. In somatic cells, CDK5RAP2 localizes throughout the pericentriolar material in all stages of the cell cycle. When overexpressed, CDK5RAP2 assembled a subset of centrosomal proteins including gamma-tubulin onto the centrosomes or under the microtubule-disrupting conditions into microtubule-nucleating clusters in the cytoplasm. CDK5RAP2 associates with the gammaTuRC via a short conserved sequence present in several related proteins found in a range of organisms from fungi to mammals. The binding of CDK5RAP2 is required for gammaTuRC attachment to the centrosome but not for gammaTuRC assembly. Perturbing CDK5RAP2 function delocalized gamma-tubulin from the centrosomes and inhibited centrosomal microtubule nucleation, thus leading to disorganization of interphase microtubule arrays and formation of anastral mitotic spindles. Together, CDK5RAP2 is a pericentriolar structural component that functions in gammaTuRC attachment and therefore in the microtubule organizing function of the centrosome. Our findings suggest that centrosome malfunction due to the CDK5RAP2 mutations may underlie autosomal recessive primary microcephaly.

MeSH Terms
Amino Acid Sequence Cell Cycle Proteins Cell Line Centrosome/metabolism,ultrastructure Conserved Sequence Humans Intracellular Signaling Peptides and Proteins/chemistry,metabolism Microtubules/metabolism Mitosis Molecular Sequence Data Nerve Tissue Proteins/chemistry,metabolism Nuclear Proteins/metabolism Protein Binding Protein Transport Spindle Apparatus/metabolism Subcellular Fractions/metabolism Tubulin/metabolism
Chemicals
CDK5RAP2 protein, human Cell Cycle Proteins Intracellular Signaling Peptides and Proteins Nerve Tissue Proteins Nuclear Proteins Tubulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fong Ka-Wing
Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Choi Yuk-Kwan
Rattner Jerome B
Qi Robert Z
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
Published
2008-01-00
Epub
2007-00-24
Pages
115-25
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2174194
Subset
IM
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