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PMID: 22184200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis.

The Journal of cell biology ·Vol. 195 ·No. 7 ·2011-12-26 ·Pages 1093-101

Lee K, Rhee K

Abstract

The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in microtubule-organizing activity. It is known that PLK1 is critical for the initiation of centrosome maturation. In this paper, we report that pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. Phosphoresistant point mutants of PCNT did not recruit centrosomal proteins, such as CEP192, GCP-WD (γ-complex protein with WD repeats), γ-tubulin, Aurora A, and PLK1, into the centrosome during mitosis. However, centrosomal recruitment of CEP215 depended on PCNT irrespective of its phosphorylation status. Furthermore, ectopic expression of PLK1-PCNT fusion proteins induced the centrosomal accumulation of CEP192, GCP-WD, and γ-tubulin even in interphase cells, mimicking centrosome maturation. Based on these results, we propose that PLK1-mediated phosphorylation of PCNT initiates centrosome maturation by organizing the spindle pole-specific PCM lattice.

MeSH Terms
Antigens/chemistry,metabolism Cell Cycle Proteins/metabolism Cells, Cultured Centrosome/metabolism HeLa Cells Humans Interphase Microtubules/metabolism Mitosis Models, Biological Phosphorylation Protein Isoforms/chemistry,metabolism Protein Serine-Threonine Kinases/metabolism Proto-Oncogene Proteins/metabolism Recombinant Fusion Proteins/metabolism Spindle Apparatus/metabolism
Chemicals
Antigens Cell Cycle Proteins Protein Isoforms Proto-Oncogene Proteins Recombinant Fusion Proteins pericentrin Protein Serine-Threonine Kinases polo-like kinase 1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee Kwanwoo
Department of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
Rhee Kunsoo
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.201106093
Published
2011-12-26
Epub
2011-00-19
Pages
1093-101
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC3246884
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.201106093 PMC Full Text View journal details
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