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PMID: 21989386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast.

The EMBO journal ·Vol. 31 ·No. 1 ·2012-01-04 ·Pages 124-37

de Castro E, Soriano I, Marín L, Serrano R, Quintales L, Antequera F

Abstract

In Schizosaccharomyces pombe, DNA replication origins (ORIs) and meiotic recombination hotspots lack consensus sequences and show a bias towards mapping to large intergenic regions (IGRs). To explore whether this preference depended on underlying chromatin features, we have generated genome-wide nucleosome profiles during mitosis and meiosis. We have found that meiotic double-strand break sites (DSBs) colocalize with nucleosome-depleted regions (NDRs) and that large IGRs include clusters of NDRs that overlap with almost half of all DSBs. By contrast, ORIs do not colocalize with NDRs and they are regulated independently of DSBs. Physical relocation of NDRs at ectopic loci or modification of their genomic distribution during meiosis was paralleled by the generation of new DSB sites. Over 80% of all meiotic DSBs colocalize with NDRs that are also present during mitosis, indicating that the recombination pattern is largely dependent on constitutive properties of the genome and, to a lesser extent, on the transcriptional profile during meiosis. The organization of ORIs and of DSBs regions in S. pombe reveals similarities and differences relative to Saccharomyces cerevisiae.

MeSH Terms
DNA Breaks, Double-Stranded Genome, Fungal Meiosis Nucleosomes/metabolism Recombination, Genetic Replication Origin Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism
Chemicals
Nucleosomes Schizosaccharomyces pombe Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Castro Elisa
Instituto de Biología Funcional y Genómica, CSIC/Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, Salamanca, Spain.
Soriano Ignacio
Marín Laura
Serrano Rebeca
Quintales Luis
Antequera Francisco
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2011.350
Published
2012-01-04
Epub
2011-00-11
Pages
124-37
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3252569
Subset
IM
External Links
PubMed source DOI: 10.1038/emboj.2011.350 PMC Full Text View journal details
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