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PMID: 15545638 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A natural meiotic DNA break site in Schizosaccharomyces pombe is a hotspot of gene conversion, highly associated with crossing over.

Genetics ·Vol. 169 ·No. 2 ·2005-02-00 ·Pages 595-605

Cromie GA, Rubio CA, Hyppa RW, Smith GR

Abstract

In Schizosaccharomyces pombe, meiosis-specific DNA breaks that initiate recombination are observed at prominent but widely separated sites. We investigated the relationship between breakage and recombination at one of these sites, the mbs1 locus on chromosome I. Breaks corresponding to 10% of chromatids were mapped to four clusters spread over a 2.1-kb region. Gene conversion of markers within the clusters occurred in 11% of tetrads (3% of meiotic chromatids), making mbs1 a conversion hotspot when compared to other fission yeast markers. Approximately 80% of these conversions were associated with crossing over of flanking markers, suggesting a strong bias in meiotic break repair toward the generation of crossovers. This bias was observed in conversion events at three other loci, ade6, ade7, and ura1. A total of 50-80% of all crossovers seen in a 90-kb region flanking mbs1 occurred in a 4.8-kb interval containing the break sites. Thus, mbs1 is also a hotspot of crossing over, with breakage at mbs1 generating most of the crossovers in the 90-kb interval. Neither Rec12 (Spo11 ortholog) nor I-SceI-induced breakage at mbs1 was significantly associated with crossing over in an apparently break-free interval >25 kb away. Possible mechanisms for generating crossovers in such break-free intervals are discussed.

MeSH Terms
Chromatids Chromosome Breakage Chromosomes, Fungal Crossing Over, Genetic DNA, Fungal Gene Conversion Genes, Fungal Genetic Markers Meiosis Models, Genetic Restriction Mapping Schizosaccharomyces/genetics
Chemicals
DNA, Fungal Genetic Markers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cromie Gareth A
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
Rubio Claudia A
Hyppa Randy W
Smith Gerald R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.037176
Published
2005-02-00
Epub
2004-00-15
Pages
595-605
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449127
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031693 · United States
NIGMS NIH HHS · R56 GM031693 · United States
NIGMS NIH HHS · GM31693 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.037176 PMC Full Text View journal details
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