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PMID: 14765111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast.

The EMBO journal ·Vol. 23 ·No. 4 ·2004-02-25 ·Pages 930-8

Kaykov A, Holmes AM, Arcangioli B

Abstract

Mating-type switching in the fission yeast Schizosaccharomyces pombe is initiated by a strand-specific imprint located at the mating-type (mat1) locus. We show that the imprint corresponds to a single-strand DNA break (SSB), which is site- but not sequence-specific. We identified three novel cis-acting elements, involved in the formation and stability of the SSB. One of these elements is essential for a replication fork pause next to mat1 and interacts in vivo with the Swi1 protein. Another element is essential for maintaining the SSB during cell cycle progression. These results suggest that the DNA break appears during the S-phase and is actively protected against repair. Consequently, during the following round of replication, a polar double-strand break is formed. We show that when the replication fork encounters the SSB, the leading-strand DNA polymerase is able to synthesize DNA to the edge of the SSB, creating a blunt-ended recombination intermediate.

MeSH Terms
Cell Cycle/genetics Cell Cycle Proteins Chromatin Immunoprecipitation DNA Damage DNA Replication/genetics DNA, Fungal/genetics DNA, Single-Stranded/genetics DNA-Binding Proteins DNA-Directed DNA Polymerase/genetics Electrophoresis, Gel, Two-Dimensional Genomic Imprinting Mutation Recombination, Genetic Regulatory Sequences, Nucleic Acid Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Transcription Factors/genetics
Chemicals
Cell Cycle Proteins DNA, Fungal DNA, Single-Stranded DNA-Binding Proteins Schizosaccharomyces pombe Proteins Transcription Factors swi1 protein, S pombe DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaykov Atanas
Unité de Dynamique du Génome, URA 1644 Département de la Structure et Dynamique des Génomes, Institut Pasteur, Paris, France.
Holmes Allyson M
Arcangioli Benoit
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600099
Published
2004-02-25
Epub
2004-00-12
Pages
930-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC381007
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600099 PMC Full Text View journal details
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