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PMID: 11156979 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae.

Genetics ·Vol. 157 ·No. 2 ·2001-02-00 ·Pages 567-77

Foss EJ

Abstract

A tof1 mutant was recovered in a screen aimed at identifying genes involved specifically in the S phase branch of the MEC1-dependent DNA damage response pathway. The screen was based on the observation that mutants missing this branch are particularly dependent on the cell cycle-wide branch and, therefore, on RAD9, for surviving DNA damage. tof1 and rad9 conferred synergistic sensitivity to MMS, UV, and HU, and the double mutant was incapable of slowing S phase in response to MMS, inducing RNR3 transcription in response to UV, and phosphorylating Rad53p in response to HU. TOF1's contribution to DNA damage response appeared to be restricted to S phase, since TOF1 did not contribute to UV-induced transcription during G1 or to the cdc13-1-induced block to anaphase in G2/M. I suggest a model in which Tof1p functions to link Mec1p with Rad53p.

MeSH Terms
Cell Cycle Proteins/genetics Cell Division Checkpoint Kinase 2 Cyclin B/genetics DNA Damage/genetics DNA-Binding Proteins Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Flow Cytometry Fungal Proteins/genetics,physiology Genotype Hydroxyurea Intracellular Signaling Peptides and Proteins Methyl Methanesulfonate Models, Genetic Mutagens Mutation Protein Kinases/genetics Protein Serine-Threonine Kinases S Phase/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Time Factors Transcription, Genetic/drug effects,genetics,radiation effects Ultraviolet Rays
Chemicals
Cell Cycle Proteins Cyclin B DNA-Binding Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins Mutagens Saccharomyces cerevisiae Proteins TOF1 protein, S cerevisiae rad9 protein Methyl Methanesulfonate Protein Kinases Checkpoint Kinase 2 MEC1 protein, S cerevisiae Protein Serine-Threonine Kinases RAD53 protein, S cerevisiae Hydroxyurea
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Foss E J
Division of Basic Sciences, A3-023, Fred Hutchinson Cancer Research Center, 1100 Faiorview Ave., Seattle, WA 98109-1024, USA. efoss@fred.fhcrc.org
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/157.2.567
Email
efoss@fred.fhcrc.org
Published
2001-02-00
Pages
567-77
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461533
Subset
IM
Grants
NIGMS NIH HHS · GM-11709 · United States
External Links
PubMed source DOI: 10.1093/genetics/157.2.567 PMC Full Text View journal details
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