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PMID: 9691032 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Schizosaccharomyces pombe S-phase checkpoint differentiates between different types of DNA damage.

Genetics ·Vol. 149 ·No. 4 ·1998-08-00 ·Pages 1729-37

Rhind N, Russell P

Abstract

We have identified an S-phase DNA damage checkpoint in Schizosaccharomyces pombe. This checkpoint is dependent on Rad3, the S. pombe homolog of the mammalian ATM/ATR checkpoint proteins, and Cds1. Cds1 had previously been believed to be involved only in the replication checkpoint. The requirement of Cds1 in the DNA damage checkpoint suggests that Cds1 may be a general target of S-phase checkpoints. Unlike other checkpoints, the S. pombe S-phase DNA damage checkpoint discriminates between different types of damage. UV-irradiation, which causes base modification that can be repaired during G1 and S-phase, invokes the checkpoint, while gamma-irradiation, which causes double-stranded breaks that cannot be repaired by a haploid cell if induced before replication, does not invoke the checkpoint. Because the same genes are required to respond to UV- and gamma-irradiation during G2, this discrimination may represent an active suppression of the gamma response during S-phase.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Checkpoint Kinase 2 DNA Damage DNA Helicases/genetics,metabolism DNA, Fungal/metabolism,radiation effects G1 Phase/genetics,physiology,radiation effects Gamma Rays Genes, Fungal Mutation Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases S Phase/genetics,physiology,radiation effects Saccharomyces cerevisiae Proteins Schizosaccharomyces/cytology,genetics,metabolism Schizosaccharomyces pombe Proteins Ultraviolet Rays
Chemicals
DNA, Fungal Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Protein Kinases Checkpoint Kinase 2 Cds1 protein, S pombe Protein Serine-Threonine Kinases Adenosine Triphosphatases Rad3 protein, S cerevisiae DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rhind N
Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Russell P
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/149.4.1729
Published
1998-08-00
Pages
1729-37
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460286
Subset
IM
Grants
NIGMS NIH HHS · F32 GM018503 · United States
NIGMS NIH HHS · F32 GM018503-02 · United States
External Links
PubMed source DOI: 10.1093/genetics/149.4.1729 PMC Full Text View journal details
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