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PMID: 1563350 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.

The EMBO journal ·Vol. 11 ·No. 4 ·1992-04-00 ·Pages 1343-50

al-Khodairy F, Carr AM

Abstract

We have tested mutants corresponding to 20 DNA repair genes of the fission yeast Schizosaccharomyces pombe for their ability to arrest in G2 after DNA damage. Of the mutants tested, four are profoundly defective in this damage dependent G2 arrest. In addition, these four mutants are highly sensitive to a transient inhibition of DNA synthesis by hydroxyurea. This suggests that the pathway responsible for the recognition of DNA damage and the subsequent mitotic arrest, shares many functions with the mechanism that controls the dependency of mitosis on the completion of S phase. The phenotype of these checkpoint rad mutants in wee mutant backgrounds indicate that the G2 arrest response is mediated either through, or in parallel with, the activity of the cdc2 gene product.

Related Genes
rad
MeSH Terms
Cell Cycle/genetics Crosses, Genetic DNA Damage DNA Repair/drug effects G2 Phase/drug effects,genetics Hydroxyurea/pharmacology Kinetics Mutation Schizosaccharomyces/drug effects,genetics,growth & development Time Factors
Chemicals
Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
al-Khodairy F
MRC Cell Mutation Unit, Sussex University, Falmer, UK.
Carr A M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-04-00
Pages
1343-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556583
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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