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PMID: 1316273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation.

The EMBO journal ·Vol. 11 ·No. 5 ·1992-05-00 ·Pages 1773-84

Tyers M, Tokiwa G, Nash R, Futcher B

Abstract

In Saccharomyces cerevisiae, several of the proteins involved in the Start decision have been identified; these include the Cdc28 protein kinase and three cyclin-like proteins, Cln1, Cln2 and Cln3. We find that Cln3 is a very unstable, low abundance protein. In contrast, the truncated Cln3-1 protein is stable, suggesting that the PEST-rich C-terminal third of Cln3 is necessary for rapid turnover. Cln3 associates with Cdc28 to form an active kinase complex that phosphorylates Cln3 itself and a co-precipitated substrate of 45 kDa. The cdc34-2 allele, which encodes a defective ubiquitin conjugating enzyme, dramatically increases the kinase activity associated with Cln3, but does not affect the half-life of Cln3. The Cln--Cdc28 complex is inactivated by treatment with non-specific phosphatases; prolonged incubation with ATP restores kinase activity to the dephosphorylated kinase complex. It is thus possible that phosphate residues essential for Cln-Cdc28 kinase activity are added autocatalytically. The multiple post-translational controls on Cln3 activity may help Cln3 tether division to growth.

Related Genes
CDC34 CLN1 CLN2 CLN3 cdc34-2
MeSH Terms
Base Sequence Blotting, Northern Blotting, Western CDC2 Protein Kinase/metabolism CDC28 Protein Kinase, S cerevisiae Cell Cycle Enzyme Stability Hydrolysis Molecular Sequence Data Phosphorylation Precipitin Tests Protein Kinases/genetics,metabolism Protein Processing, Post-Translational RNA, Messenger/metabolism Saccharomyces cerevisiae/cytology,enzymology,genetics Ubiquitins/metabolism
Chemicals
RNA, Messenger Ubiquitins Protein Kinases CDC2 Protein Kinase CDC28 Protein Kinase, S cerevisiae Cln3-Cdc28 kinase complex, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tyers M
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724.
Tokiwa G
Nash R
Futcher B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-05-00
Pages
1773-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556635
Subset
IM
Grants
NIGMS NIH HHS · GM39978 · United States
External Links
PubMed source PMC Full Text View journal details
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