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PMID: 7862159 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans.

Molecular and cellular biology ·Vol. 15 ·No. 3 ·1995-03-00 ·Pages 1679-88

Fan Q, Xu F, Petes TD

Abstract

The region of Saccharomyces cerevisiae chromosome III located between the 5' end of the HIS4 gene and the 3' end of the adjacent BIK1 gene has a very high level of meiotic recombination. In wild-type strains, a meiosis-specific double-strand DNA break occurs in the hot spot region. This break is absent in strains in which the transcription factors Rap1p, Bas1p, and Bas2p cannot bind to the region upstream of HIS4. In strains with levels of recombination that are higher than those of the wild type, the break is found at elevated levels. The linear relationship between hot spot activity and the frequency of double-strand DNA breaks suggests that these lesions are responsible for initiating recombination at the HIS4 recombination hot spot.

Related Genes
BIK1 HIS4
MeSH Terms
Chromosome Aberrations Chromosomes, Fungal DNA, Fungal/metabolism Genotype Haploidy Meiosis Plasmids Recombination, Genetic Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/cytology,genetics,metabolism Species Specificity Transcription Factors/metabolism
Chemicals
DNA, Fungal Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fan Q
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Xu F
Petes T D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.3.1679
Published
1995-03-00
Pages
1679-88
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230392
Subset
IM
Grants
NIGMS NIH HHS · GM24110 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.3.1679 PMC Full Text View journal details
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