Home LiteratureArticle Details
PMID: 7991560 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Initiation of meiotic recombination is independent of interhomologue interactions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 25 ·1994-12-06 ·Pages 11934-7

Gilbertson LA, Stahl FW

Abstract

In yeast meiosis, crossing-over between homologues is dependent upon double-strand breaks. We demonstrate that the occurrence of these breaks is independent of pairing between homologues by showing that they occur with normal frequency, timing, and position in the absence of a homologue. This observation supports models that view double-strand breaks as initiating events and crossing-over as a consequence of repair of these breaks.

MeSH Terms
Chromosomes, Fungal DNA, Fungal/analysis,metabolism Kinetics Meiosis Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics Species Specificity Spores, Fungal/physiology Time Factors
Chemicals
DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilbertson L A
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229.
Stahl F W
References (23)
23 references, click to expand
  1. Meiotic chromosome synapsis in a haploid yeast.
    Chromosoma. 1991 May;100(4):221-8 PMID: 2055133
  2. Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast.
    Genetics. 1990 Dec;126(4):813-22 PMID: 1981763
  3. Genetic and physical analyses of sister chromatid exchange in yeast meiosis.
    Mol Cell Biol. 1991 Dec;11(12):6328-36 PMID: 1944292
  4. DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9755-9 PMID: 1946399
  5. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
    Cell. 1992 May 1;69(3):439-56 PMID: 1581960
  6. Meiotic chromosome metabolism: one view.
    Cold Spring Harb Symp Quant Biol. 1991;56:729-43 PMID: 1819520
  7. Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase.
    Mol Cell Biol. 1993 Jan;13(1):373-82 PMID: 8417336
  8. Yeast genetics and the fall of the classical view of meiosis.
    Cell. 1993 Feb 12;72(3):301-3 PMID: 8431941
  9. A short chromosomal region with major roles in yeast chromosome III meiotic disjunction, recombination and double strand breaks.
    Genetics. 1993 Feb;133(2):159-69 PMID: 8436266
  10. Meiosis-induced double-strand break sites determined by yeast chromatin structure.
    Science. 1994 Jan 28;263(5146):515-8 PMID: 8290959
  11. Homologous pairing is reduced but not abolished in asynaptic mutants of yeast.
    J Cell Biol. 1994 Jun;125(6):1191-200 PMID: 8207053
  12. Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast.
    Cell. 1994 Jul 1;77(7):977-91 PMID: 8020104
  13. Carbohydrate metabolism during ascospore development in yeast.
    J Bacteriol. 1974 Apr;118(1):8-14 PMID: 4595206
  14. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  15. Characterization of two genes required for the position-effect control of yeast mating-type genes.
    EMBO J. 1984 Dec 1;3(12):2817-23 PMID: 6098447
  16. Meiotic exchange within and between chromosomes requires a common Rec function in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Dec;5(12):3532-44 PMID: 3915779
  17. Developmental regulation of SPO13, a gene required for separation of homologous chromosomes at meiosis I.
    Mol Cell Biol. 1987 Apr;7(4):1425-35 PMID: 3299047
  18. An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.
    Nature. 1989 Mar 2;338(6210):35-9 PMID: 2537472
  19. Double-strand breaks at an initiation site for meiotic gene conversion.
    Nature. 1989 Mar 2;338(6210):87-90 PMID: 2645528
  20. Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination.
    Cell. 1990 May 4;61(3):419-36 PMID: 2185891
  21. A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae.
    Cell. 1990 Jun 15;61(6):1089-101 PMID: 2190690
  22. Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.
    Cell. 1991 Mar 22;64(6):1155-61 PMID: 2004421
  23. Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae.
    Cell. 1991 Sep 20;66(6):1239-56 PMID: 1913808
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.25.11934
Published
1994-12-06
Pages
11934-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45350
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.25.11934 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2