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PMID: 1324174 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Multiple sites for double-strand breaks in whole meiotic chromosomes of Saccharomyces cerevisiae.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3441-7

Zenvirth D, Arbel T, Sherman A, Goldway M, Klein S, Simchen G

Abstract

We present a scheme for locating double-strand breaks (DSBs) in meiotic chromosomes of Saccharomyces cerevisiae, based on the separation of large DNA molecules by pulsed field gel electrophoresis. Using a rad50S mutant, in which DSBs are not processed, we show that DSBs are widely induced in S. cerevisiae chromosomes during meiosis. Some of the DSBs accumulate at certain preferred sites. We present general profiles of DSBs in chromosomes III, V, VI and VII. A map of the 12 preferred sites on chromosome III is presented. At least some of these sites correlate with known 'hot spots' for meiotic recombination. The data are discussed in view of current models of meiotic recombination and chromosome segregation.

MeSH Terms
Chromosome Aberrations Chromosome Mapping Chromosomes, Fungal DNA Restriction Enzymes DNA, Fungal/genetics Electrophoresis, Gel, Pulsed-Field Meiosis/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zenvirth D
Department of Genetics, Hebrew University of Jerusalem, Israel.
Arbel T
Sherman A
Goldway M
Klein S
Simchen G
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-09-00
Pages
3441-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556879
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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