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PMID: 15466419 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe.

Genetics ·Vol. 168 ·No. 4 ·2004-12-00 ·Pages 1891-8

Ellermeier C, Schmidt H, Smith GR

Abstract

Previously isolated Schizosaccharomyces pombe swi5 mutants are defective in mitotic mating-type switching and in repair of meiotic recombination-related DNA double-strand breaks. Here, we identify the swi5 gene, which encodes an 85-amino-acid polypeptide, similar to Sae3 of Saccharomyces cerevisiae, with an N-terminal predicted coiled-coil domain. A swi5 complete deletion mutant had normal mitotic growth rate but was hypersensitive to DNA-damaging agents and defective in mating-type switching. In meiosis, recombinant frequencies were reduced by a factor of approximately 10. The swi5 deletion strongly reduced the viable spore yields of mutants lacking Rhp55 or Rhp57, proteins thought to aid joint molecule formation. Furthermore, the swi5 deletion strongly suppressed the low viable spore yield of mutants lacking Mus81*Eme1, which resolves joint molecules such as Holliday junctions. These and previous results indicate that the small Swi5 polypeptide acts in a branched pathway of joint molecule formation to repair meiotic DNA breaks.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Sequence Cloning, Molecular DNA/metabolism DNA-Binding Proteins/genetics,metabolism Endonucleases/genetics,metabolism Meiosis/genetics Molecular Sequence Data Mutation Phenotype Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism Sequence Analysis, DNA Spores, Fungal/genetics,metabolism
Chemicals
DNA-Binding Proteins MUS81 protein, S pombe RHP55 protein, S pombe Schizosaccharomyces pombe Proteins Swi5 protein, S pombe DNA Endonucleases Adenosine Triphosphatases Rhp57 protein, S pombe
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ellermeier Chad
Fred Hutchinson Research Cancer Center, Seattle, Washington 98109, USA.
Schmidt Henning
Smith Gerald R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.034280
Published
2004-12-00
Epub
2004-00-30
Pages
1891-8
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1448720
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032194 · United States
NIGMS NIH HHS · GM-32194 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.034280 PMC Full Text View journal details
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