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PMID: 10077566 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 6 ·1999-03-16 ·Pages 2656-61

Chuang RY, Kelly TJ

Abstract

The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. Although ORC appears to play an essential role in the initiation of DNA replication in the cells of all eukaryotes, its interactions with DNA have not been defined in species other than budding yeast. We have characterized a Schizosaccharomyces pombe homologue of the ORC subunit, Orc4p. The homologue (Orp4p) consists of two distinct functional domains. The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. The N-terminal domain contains nine copies of the AT-hook motif found in a number of DNA-binding proteins, including the members of the HMG-I(Y) family of chromatin proteins. AT-hook motifs are known from biochemical and structural studies to mediate binding to the minor groove of AT-tracts in DNA. Orp4p is essential for viability of Sc. pombe and is expressed throughout the cell cycle. The Orp4 protein (and its isolated N-terminal domain) binds to the Sc. pombe replication origin, ars1. The DNA binding properties of Orp4p provide a plausible explanation for the characteristic features of Sc. pombe origins of replication, which differ significantly from those of Sa. cerevisiae.

MeSH Terms
Amino Acid Sequence DNA Replication DNA, Fungal/genetics DNA-Binding Proteins/genetics Fungal Proteins/genetics Humans Molecular Sequence Data Origin Recognition Complex Replication Origin/genetics Repressor Proteins/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Origin Recognition Complex Repressor Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chuang R Y
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21210, USA.
Kelly T J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.6.2656
Published
1999-03-16
Pages
2656-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC15824
Subset
IM
Databases
GENBANK
AF125185
External Links
PubMed source DOI: 10.1073/pnas.96.6.2656 PMC Full Text View journal details
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