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PMID: 8248139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

De novo synthesis of budding yeast DNA polymerase alpha and POL1 transcription at the G1/S boundary are not required for entrance into S phase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 22 ·1993-11-15 ·Pages 10519-23

Muzi Falconi M, Piseri A, Ferrari M, Lucchini G, Plevani P, Foiani M

Abstract

The POL1 gene, encoding DNA polymerase alpha (pol alpha) in Saccharomyces cerevisiae, is transiently transcribed during the cell cycle at the G1/S phase boundary. Here we show that yeast pol alpha is present at every stage of the cell cycle, and its level only slightly increases following the peak of POL1 transcription. POL1 mRNA synthesis driven by a GAL1 promoter can be completely abolished without affecting the growth rate of logarithmically growing yeast cultures for several cell divisions, although the amount of the pol alpha polypeptide drops below the physiological level. Moreover, alpha-factor-arrested cells can enter S phase and divide synchronously even if POL1 transcription is abolished. These results indicate that the level of yeast pol alpha is not rate limiting and de novo synthesis of the enzyme is not required for entrance into S phase.

Related Genes
CMD1 H2A POL1 PRI2
MeSH Terms
Cell Cycle DNA Polymerase II/biosynthesis,genetics Gene Expression Regulation, Fungal Genes, Fungal RNA, Fungal/genetics RNA, Messenger/genetics S Phase Saccharomyces cerevisiae/cytology,enzymology,genetics
Chemicals
RNA, Fungal RNA, Messenger DNA Polymerase II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Muzi Falconi M
Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
Piseri A
Ferrari M
Lucchini G
Plevani P
Foiani M
References (39)
39 references, click to expand
  1. Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.
    Curr Genet. 1986;11(2):107-12 PMID: 3329047
  2. Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase.
    Genes Dev. 1991 Sep;5(9):1589-600 PMID: 1885001
  3. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1440-8 PMID: 6092912
  4. Initiation of simian virus 40 DNA synthesis in vitro.
    Mol Cell Biol. 1991 May;11(5):2350-61 PMID: 1673224
  5. Cell cycle-dependent phosphorylation of human DNA polymerase alpha.
    J Biol Chem. 1991 Apr 25;266(12):7893-903 PMID: 1902230
  6. GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Jun;11(6):3203-16 PMID: 2038326
  7. A role for the nuclear envelope in controlling DNA replication within the cell cycle.
    Nature. 1988 Apr 7;332(6164):546-8 PMID: 3357511
  8. DNA polymerase alpha in the fission yeast Schizosaccharomyces pombe: identification and tracing of the catalytic subunit during the cell cycle.
    Exp Cell Res. 1992 Feb;198(2):183-90 PMID: 1729129
  9. Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.
    Mol Cell Biol. 1988 Nov;8(11):5016-25 PMID: 3211134
  10. Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.
    EMBO J. 1992 Dec;11(13):5111-20 PMID: 1361173
  11. DNA polymerase I gene of Saccharomyces cerevisiae: nucleotide sequence, mapping of a temperature-sensitive mutation, and protein homology with other DNA polymerases.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3772-6 PMID: 3287376
  12. Positive cis-acting regulatory sequences mediate proper control of POL1 transcription in Saccharomyces cerevisiae.
    Curr Genet. 1992 Mar;21(3):183-9 PMID: 1563043
  13. CLB5: a novel B cyclin from budding yeast with a role in S phase.
    Genes Dev. 1992 Sep;6(9):1695-706 PMID: 1387626
  14. The isolated 48,000-dalton subunit of yeast DNA primase is sufficient for RNA primer synthesis.
    J Biol Chem. 1993 Jan 15;268(2):1343-8 PMID: 7678254
  15. Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex.
    J Biol Chem. 1985 Jun 10;260(11):7102-7 PMID: 3888995
  16. Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.
    Genes Dev. 1990 Jun;4(6):968-77 PMID: 2200738
  17. Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division.
    EMBO J. 1992 Jun;11(6):2167-76 PMID: 1600944
  18. A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast.
    Nature. 1992 Jun 11;357(6378):508-13 PMID: 1608451
  19. The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.
    Nucleic Acids Res. 1987 Jul 10;15(13):5017-30 PMID: 3299263
  20. Mammalian DNA ligases. Biosynthesis and intracellular localization of DNA ligase I.
    J Biol Chem. 1990 Jul 25;265(21):12618-22 PMID: 2197279
  21. Regulation of proliferating cell nuclear antigen during the cell cycle.
    J Biol Chem. 1989 Aug 15;264(23):13856-64 PMID: 2569465
  22. Subcellular localization of yeast CDC46 varies with the cell cycle.
    Genes Dev. 1990 Dec;4(12B):2252-63 PMID: 2279699
  23. Periodic transcription as a means of regulating gene expression during the cell cycle: contrasting modes of expression of DNA ligase genes in budding and fission yeast.
    EMBO J. 1986 Jul;5(7):1705-9 PMID: 3527694
  24. Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.
    Nature. 1990 Aug 9;346(6284):534-9 PMID: 2165567
  25. SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast.
    Nature. 1992 Jun 11;357(6378):505-8 PMID: 1608450
  26. Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.
    Genes Dev. 1992 Nov;6(11):2021-34 PMID: 1427070
  27. Turning DNA replication on and off.
    Curr Opin Cell Biol. 1993 Apr;5(2):201-6 PMID: 8507492
  28. Mechanism of activation of simian virus 40 DNA replication by protein phosphatase 2A.
    Mol Cell Biol. 1992 Nov;12(11):4883-95 PMID: 1328866
  29. Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.
    J Biol Chem. 1991 Feb 15;266(5):3005-15 PMID: 1704371
  30. Eukaryotic DNA polymerases.
    Annu Rev Biochem. 1991;60:513-52 PMID: 1883203
  31. The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast.
    Cell. 1991 Sep 6;66(5):995-1013 PMID: 1832338
  32. Animal cell cycles and their control.
    Annu Rev Biochem. 1992;61:441-70 PMID: 1497317
  33. A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Apr;12(4):1879-92 PMID: 1312673
  34. Cell cycle control of gene expression in yeast.
    Trends Cell Biol. 1992 Dec;2(12):353-7 PMID: 14731955
  35. Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9479-83 PMID: 1409658
  36. Cell cycle control of DNA synthesis in budding yeast.
    Nucleic Acids Res. 1992 May 25;20(10):2403-10 PMID: 1598198
  37. A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Jul;9(7):3081-7 PMID: 2528682
  38. Fluorescence microscopy methods for yeast.
    Methods Cell Biol. 1989;31:357-435 PMID: 2476649
  39. A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase alpha in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6058-62 PMID: 2068085
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.22.10519
Published
1993-11-15
Pages
10519-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47808
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.90.22.10519 PMC Full Text View journal details
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