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PMID: 16428454 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 26 ·No. 3 ·2006-02-00 ·Pages 1014-27

Mieczkowski PA, Dominska M, Buck MJ, Gerton JL, Lieb JD, Petes TD

Abstract

In the yeast Saccharomyces cerevisiae, certain genomic regions have very high levels of meiotic recombination (hot spots). The hot spot activity associated with the HIS4 gene requires the Bas1p transcription factor. To determine whether this relationship between transcription factor binding and hot spot activity is general, we used DNA microarrays to map all genomic Bas1p binding sites and to map the frequency of meiosis-specific double-strand DNA breaks (as an estimate of the recombination activity) of all genes in both wild-type and bas1 strains. We identified sites of Bas1p-DNA interactions upstream of 71 genes, many of which are involved in histidine and purine biosynthesis. Our analysis of recombination activity in wild-type and bas1 strains showed that the recombination activities of some genes with Bas1p binding sites were dependent on Bas1p (as observed for HIS4), whereas the activities of other genes with Bas1p binding sites were unaffected or were repressed by Bas1p. These data demonstrate that the effect of transcription factors on meiotic recombination activity is strongly context dependent. In wild-type and bas1 strains, meiotic recombination was strongly suppressed in large (25- to 150-kb) chromosomal regions near the telomeres and centromeres and in the region flanking the rRNA genes. These results argue that both local and regional factors affect the level of meiotic recombination.

MeSH Terms
Binding Sites Chromosomes, Fungal/genetics,metabolism DNA Damage DNA, Fungal/metabolism Diploidy Genes, Fungal Histidine/biosynthesis,genetics Meiosis/genetics Mutation Oligonucleotide Array Sequence Analysis Purines/biosynthesis Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Trans-Activators/genetics,metabolism
Chemicals
BAS1 protein, S cerevisiae DNA, Fungal Purines Saccharomyces cerevisiae Proteins Trans-Activators Histidine purine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mieczkowski Piotr A
Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280, USA.
Dominska Margaret
Buck Michael J
Gerton Jennifer L
Lieb Jason D
Petes Thomas D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.26.3.1014-1027.2006
Published
2006-02-00
Pages
1014-27
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1347019
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024110 · United States
NIGMS NIH HHS · R01 GM072518 · United States
NIGMS NIH HHS · GM072518 · United States
NIGMS NIH HHS · GM24110 · United States
External Links
PubMed source DOI: 10.1128/MCB.26.3.1014-1027.2006 PMC Full Text View journal details
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