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PMID: 9207111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 14 ·1997-07-08 ·Pages 7446-51

Fox ME, Virgin JB, Metzger J, Smith GR

Abstract

The activity of the M26 meiotic recombination hot spot of Schizosaccharomyces pombe depends on the presence of the heptamer 5'-ATGACGT-3'. Transplacement of DNA fragments containing the ade6-M26 gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1-4 bp in the ade6 and ura4 genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for M26 hot spot activity.

MeSH Terms
DNA, Fungal/genetics Meiosis Mutagenesis, Site-Directed Recombination, Genetic Schizosaccharomyces/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fox M E
Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA.
Virgin J B
Metzger J
Smith G R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.14.7446
Published
1997-07-08
Pages
7446-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23841
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031693 · United States
NIGMS NIH HHS · R56 GM031693 · United States
NCI NIH HHS · CA08922 · United States
NIGMS NIH HHS · GM31693 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.14.7446 PMC Full Text View journal details
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