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PMID: 8662192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The distance-dependence of the fission yeast ade6-M26 marker effect in two-factor crosses.

Current genetics ·Vol. 29 ·No. 6 ·1996-05-00 ·Pages 530-6

Zahn-Zabal M, Kohli J

Abstract

Random spore analysis of crosses between a strain bearing the ade6-M26 hotspot mutation and strains bearing other ade6 mutations was performed. Recombinant prototroph frequencies increase with increasing distance from M26 for mutations both 5' and 3' of M26. Maximum prototroph frequencies are obtained for mutations lying more than 700 nucleotides downstream from M26. Similar results are obtained for crosses with the ade6-M375 control mutation, but the prototroph frequencies are lower. The factor of stimulation of recombination by M26 as compared to the M375 control (M26 marker effect) also displays distance-dependence. These results are discussed in the context of the mechanism of M26 recombination, as well as in relation to recombination initiation, hybrid DNA formation, and mismatch repair at ade6. Keywords Conversion middle dot M26 hotspot middle dot Recombination middle dot Schizosaccharomyces pombe

MeSH Terms
Alleles Crosses, Genetic DNA Repair/genetics DNA, Fungal/genetics Genes, Fungal Genetic Markers Mutation Nucleic Acid Heteroduplexes/genetics Recombination, Genetic Schizosaccharomyces/genetics Spores, Fungal/genetics
Chemicals
DNA, Fungal Genetic Markers Nucleic Acid Heteroduplexes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zahn-Zabal M
Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012 Bern, Switzerland.
Kohli J
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF02426957
Published
1996-05-00
Pages
530-6
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02426957 View journal details
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