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PMID: 9144217 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Clustering of meiotic double-strand breaks on yeast chromosome III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 10 ·1997-05-13 ·Pages 5213-8

Baudat F, Nicolas A

Abstract

In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by transient DNA double-strand breaks (DSBs) that are repaired by interaction of the broken chromosome with its homologue. To identify a large number of DSB sites and gain insight into the control of DSB formation at both the local and the whole chromosomal levels, we have determined at high resolution the distribution of meiotic DSBs along the 340 kb of chromosome III. We have found 76 DSB regions, mostly located in intergenic promoter-containing intervals. The frequency of DSBs varies at least 50-fold from one region to another. The global distribution of DSB regions along chromosome III is nonrandom, defining large (39-105 kb) chromosomal domains, both hot and cold. The distribution of these localized DSBs indicates that they are likely to initiate most crossovers along chromosome III, but some discrepancies remain to be explained.

MeSH Terms
Chromosome Mapping Chromosomes, Fungal Crossing Over, Genetic DNA Transposable Elements DNA, Fungal/genetics Meiosis/genetics Open Reading Frames Plasmids Restriction Mapping Saccharomyces cerevisiae/cytology,genetics
Chemicals
DNA Transposable Elements DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baudat F
Institut Curie, Section de Recherche, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, Compartimentation et Dynamique Cellulaires, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Nicolas A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.10.5213
Published
1997-05-13
Pages
5213-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24658
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.10.5213 PMC Full Text View journal details
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