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PMID: 20351051 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Conserved nucleosome positioning defines replication origins.

Genes & development ·Vol. 24 ·No. 8 ·2010-04-15 ·Pages 748-53

Eaton ML, Galani K, Kang S, Bell SP, MacAlpine DM

Abstract

The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.

MeSH Terms
Chromosomes, Fungal/genetics Genome, Fungal/genetics Nucleosomes/genetics Replication Origin/genetics Saccharomyces cerevisiae/genetics Sequence Analysis
Chemicals
Nucleosomes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Eaton Matthew L
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Galani Kyriaki
Kang Sukhyun
Bell Stephen P
MacAlpine David M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.1913210
Published
2010-04-15
Epub
2010-00-29
Pages
748-53
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC2854390
Subset
IM
Grants
NHGRI NIH HHS · U01 HG004279 · United States
NHGRI NIH HHS · HG004279 · United States
External Links
PubMed source DOI: 10.1101/gad.1913210 PMC Full Text View journal details
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