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PMID: 20847746 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Advances in understanding cancer genomes through second-generation sequencing.

Nature reviews. Genetics ·Vol. 11 ·No. 10 ·2010-10-00 ·Pages 685-96

Meyerson M, Gabriel S, Getz G

Abstract

Cancers are caused by the accumulation of genomic alterations. Therefore, analyses of cancer genome sequences and structures provide insights for understanding cancer biology, diagnosis and therapy. The application of second-generation DNA sequencing technologies (also known as next-generation sequencing) - through whole-genome, whole-exome and whole-transcriptome approaches - is allowing substantial advances in cancer genomics. These methods are facilitating an increase in the efficiency and resolution of detection of each of the principal types of somatic cancer genome alterations, including nucleotide substitutions, small insertions and deletions, copy number alterations, chromosomal rearrangements and microbial infections. This Review focuses on the methodological considerations for characterizing somatic genome alterations in cancer and the future prospects for these approaches.

MeSH Terms
Computational Biology/methods Exons/genetics Gene Expression Profiling/methods Genome, Human/genetics Genome-Wide Association Study/methods Genomics/methods Humans Mutation Neoplasms/genetics,pathology Sequence Analysis, DNA/methods
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meyerson Matthew
Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. matthew_meyerson@dfci.harvard.edu
Gabriel Stacey
Getz Gad
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Article Info
Journal
Nature reviews. Genetics
Abbr.
Nat Rev Genet
ISSN
1471-0064
DOI
10.1038/nrg2841
Email
matthew_meyerson@dfci.harvard.edu
Published
2010-10-00
Pages
685-96
Language
English
Region
England
NLM ID
100962779
Subset
IM
Grants
NCI NIH HHS · U24CA143845 · United States
NCI NIH HHS · U24CA143867 · United States
NHGRI NIH HHS · U54HG003067 · United States
External Links
PubMed source DOI: 10.1038/nrg2841 View journal details
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