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PMID: 12461184 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Digital karyotyping.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 25 ·2002-12-10 ·Pages 16156-61

Wang TL, Maierhofer C, Speicher MR, Lengauer C, Vogelstein B, Kinzler KW, Velculescu VE

Abstract

Alterations in the genetic content of a cell are the underlying cause of many human diseases, including cancers. We have developed a method, called digital karyotyping, that provides quantitative analysis of DNA copy number at high resolution. This approach involves the isolation and enumeration of short sequence tags from specific genomic loci. Analysis of human cancer cells by using this method identified gross chromosomal changes as well as amplifications and deletions, including regions not previously known to be altered. Foreign DNA sequences not present in the normal human genome could also be readily identified. Digital karyotyping provides a broadly applicable means for systematic detection of DNA copy number changes on a genomic scale.

MeSH Terms
Analog-Digital Conversion Biotinylation Chromosome Mapping Chromosomes, Human/genetics Colorectal Neoplasms/genetics Computer Simulation DNA Restriction Enzymes DNA, Neoplasm/genetics DNA, Viral/analysis,genetics Gene Dosage Genetic Markers Herpesvirus 4, Human/genetics Humans Karyotyping/methods Microspheres Monte Carlo Method Sensitivity and Specificity Streptavidin Templates, Genetic Tumor Cells, Cultured
Chemicals
DNA, Neoplasm DNA, Viral Genetic Markers Streptavidin DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Tian-Li
The Howard Hughes Medical Institute and The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA.
Maierhofer Christine
Speicher Michael R
Lengauer Christoph
Vogelstein Bert
Kinzler Kenneth W
Velculescu Victor E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.202610899
Published
2002-12-10
Epub
2002-00-02
Pages
16156-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC138581
Subset
IM
Grants
NCI NIH HHS · CA57345 · United States
NCI NIH HHS · R37 CA043460 · United States
NCI NIH HHS · CA43460 · United States
NCI NIH HHS · R37 CA057345 · United States
NCI NIH HHS · CA6292 · United States
NCI NIH HHS · R01 CA057345 · United States
External Links
PubMed source DOI: 10.1073/pnas.202610899 PMC Full Text View journal details
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