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PMID: 2062642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Promoter function and structure of the growth factor-inducible immediate early gene cyr61.

Nucleic acids research ·Vol. 19 ·No. 12 ·1991-06-25 ·Pages 3261-7

Latinkic BV, O'Brien TP, Lau LF

Abstract

cyr61 is an immediate early gene that is transcriptionally activated in 3T3 fibroblasts by serum, platelet-derived growth factor, fibroblast growth factor, and the tumor promoter TPA with kinetics similar to the induction of c-fos. cyr61 encodes a secreted protein that is associated with the cell surface and the extracellular matrix, and may play a role in cell-cell communication. We report here the complete nucleotide sequence of the mouse cyr61 gene, which contains four short introns. The transcription start site was mapped by S1 nuclease and primer extension analyses. A 2 kb 5' flanking DNA fragment functions as a serum-inducible promoter. This DNA fragment contains a poly(CA) sequence that can adopt the Z DNA form. In addition, it contains a sequence that resembles the serum response element (SRE) originally identified in the c-fos promoter. We show that deletion of the cry61 SRE-like sequence abrogates serum inducibility. Furthermore, this SRE-like sequence is sufficient to confer serum and growth factor inducibility when linked to a basal promoter, and binds the 67 kD serum response factor in vitro. We conclude that the cyr61 SRE functions as a serum response element and may account for the coordinate activation of cyr61 and c-fos.

Related Genes
cyr61
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cysteine-Rich Protein 61 DNA DNA-Binding Proteins/metabolism Fibroblast Growth Factors/pharmacology Gene Expression Regulation Growth Substances/genetics Immediate-Early Proteins Intercellular Signaling Peptides and Proteins Introns Mice Molecular Sequence Data Nuclear Proteins/metabolism Platelet-Derived Growth Factor/pharmacology Promoter Regions, Genetic Proteins/genetics Restriction Mapping Serum Response Factor Signal Transduction Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic
Chemicals
CCN1 protein, mouse Cysteine-Rich Protein 61 DNA-Binding Proteins Growth Substances Immediate-Early Proteins Intercellular Signaling Peptides and Proteins Nuclear Proteins Platelet-Derived Growth Factor Proteins Serum Response Factor Fibroblast Growth Factors DNA Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Latinkic B V
Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
O'Brien T P
Lau L F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.12.3261
Published
1991-06-25
Pages
3261-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328320
Subset
IM
Grants
NCI NIH HHS · R01 CA52220 · United States
Databases
GENBANK
X56790, X56865, X57509, X57510, X58491, X58492, X58493, X58494, X58495, X58496
External Links
PubMed source DOI: 10.1093/nar/19.12.3261 PMC Full Text View journal details
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