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PMID: 2147223 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional analysis of an isolated fos promoter element with AP-1 site homology reveals cell type-specific transcriptional properties.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6273-82

Velcich A, Ziff EB

Abstract

A DNA element located at positions -295 to -289 of the c-fos promoter (FAP site) is highly homologous to a consensus 12-O-tetradecanoyl phorbol-13-acetate-responsive element (TRE) and to a cyclic AMP (cAMP)-responsive element (CRE). We found that an oligonucleotide containing the FAP element was a transcription regulator which was distinct from both the TRE and CRE. When cloned in multiple copies in front of a reporter gene in HeLa cells, the FAP oligonucleotide was a powerful constitutive activator sequence. Conversely, in the same cells, reporter plasmids containing multiple copies of the TRE of the human metallothionein gene required phorbol esters for their induction. In PC12 cells, the FAP oligonucleotide was cAMP responsive. Its activity was mediated through a cAMP-dependent protein kinase II and did not rely on ongoing protein synthesis for activation. Adenovirus E1a proteins activated viral promoters through ATF (activation transcription factor) consensus binding sequences identical to the CRE. However, E1a repressed the FAP oligonucleotide-associated transcriptional activity in HeLa cells. In PC12 cells, E1a neither transactivated nor transrepressed the basal and cAMP-stimulated FAP activity. In contrast, the CRE of the human c-fos promoter located at -60 was weakly induced by cAMP and E1a in both HeLa and PC12 cells. We suggest that the FAP oligonucleotide acts through a factor(s) distinct from those employed by the TRE and CRE and that the FAP-associated protein factor(s) may differ in HeLa and PC12 cells in expression or posttranslational regulation.

Related Genes
fos
MeSH Terms
Base Sequence Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins/genetics Globins/genetics HeLa Cells/drug effects,metabolism Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun/genetics Proto-Oncogenes RNA, Messenger/genetics,isolation & purification Sequence Homology, Nucleic Acid Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/genetics Transcription, Genetic Transfection
Chemicals
Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins Oligonucleotide Probes Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun RNA, Messenger Transcription Factors Globins Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Velcich A
Department of Biochemistry, New York University Medical School, New York 10016.
Ziff E B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6273-6282.1990
Published
1990-12-00
Pages
6273-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362902
Subset
IM
Grants
NIGMS NIH HHS · GM-30760 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.12.6273-6282.1990 PMC Full Text View journal details
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