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PMID: 2157971 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The c-fos cyclic AMP-responsive element conveys constitutive expression to a tissue-specific promoter.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2402-6

Webster KA, Kedes L

Abstract

The c-fos and cardiac alpha-actin promoters share homologous 5' protein binding elements that are essential for serum-inducible and tissue-specific expression, respectively. Additional elements, auxiliary proteins or factor modifications, must distinguish the individual transcriptional responses of these two promoters. An element in the c-fos basal promoter that is normally responsible for transient stimulation of the fos gene in response to Ca2+ or cyclic AMP (CRE) may be able to modulate the expression of the upstream elements. We report here that this element, when inserted into the cardiac alpha-actin promoter, conveys constitutive expression to this otherwise highly restricted promoter. Additional data support the proposal that the CRE binding protein creates an alternative pathway whereby upstream regulatory elements in the cardiac alpha-actin promoter can activate transcription in a manner which circumvents the requirement for a tissue-specific environment.

MeSH Terms
Actins/genetics Base Sequence Cyclic AMP/physiology DNA Mutational Analysis DNA-Binding Proteins/physiology Gene Expression Regulation Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Regulatory Sequences, Nucleic Acid Transcription, Genetic Transfection
Chemicals
Actins DNA-Binding Proteins Oligonucleotide Probes Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Webster K A
Center for Molecular Medicine, School of Medicine, University of Southern California, Los Angeles 90033.
Kedes L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.2402-2406.1990
Published
1990-05-00
Pages
2402-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360588
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.5.2402-2406.1990 PMC Full Text View journal details
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