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PMID: 1708123 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of junB by PKC and PKA signal transduction through a novel cis-acting element.

Nucleic acids research ·Vol. 19 ·No. 4 ·1991-02-25 ·Pages 775-81

de Groot RP, Auwerx J, Karperien M, Staels B, Kruijer W

Abstract

The product of the junB gene, a gene homologous to the proto-oncogene c-jun, is a component of transcription factor AP-1. JunB expression is modulated by a wide variety of extracellular stimuli, such as serum, growth factors, phorbol esters (TPA) and activators of protein kinase A (PKA). In order to study the molecular basis of this complex regulation, we have cloned the mouse junB gene from a genomic testis library, and characterized the junB promoter. Here we show that the junB promoter is activated by serum, TPA, and activated PKA. Sequences located between -91 and -44 are necessary for induction. These sequences contain a CAAT box, a G-C rich region and a previously undescribed inverted repeat (IR). The IR element can mediate induction by TPA and PKA when coupled to a heterologous promoter, and specifically binds a protein of 110 kD.

Related Genes
junB
MeSH Terms
Animals Base Sequence Blood Blotting, Northern Cross-Linking Reagents DNA-Binding Proteins/genetics Mice Molecular Sequence Data Plasmids Promoter Regions, Genetic Protein Kinase C/metabolism Protein Kinases/metabolism Proto-Oncogene Proteins c-jun RNA/analysis Signal Transduction Transcription Factors/genetics Transfection Tumor Cells, Cultured
Chemicals
Cross-Linking Reagents DNA-Binding Proteins Proto-Oncogene Proteins c-jun Transcription Factors RNA Protein Kinases Protein Kinase C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
de Groot R P
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Auwerx J
Karperien M
Staels B
Kruijer W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.4.775
Published
1991-02-25
Pages
775-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333710
Subset
IM
Databases
GENBANK
S74822, S74824, S74828, S74831, S74923, S74924, S74925, S74926, X56666, X60103
External Links
PubMed source DOI: 10.1093/nar/19.4.775 PMC Full Text View journal details
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