Home LiteratureArticle Details
PMID: 2109185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 1931-9

Cao XM, Koski RA, Gashler A, McKiernan M, Morris CF, Gaffney R, Hay RV, Sukhatme VP

Abstract

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

MeSH Terms
Animals Base Sequence Cell Differentiation Cell Division Cloning, Molecular DNA-Binding Proteins/genetics Epidermal Growth Factor/pharmacology Gene Expression Regulation/drug effects Immunologic Techniques Metalloproteins/genetics Mice Molecular Sequence Data Nerve Growth Factors/pharmacology Nuclear Proteins/physiology Phosphoproteins/genetics Protein Processing, Post-Translational Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/genetics Zinc/physiology
Chemicals
DNA-Binding Proteins Metalloproteins Nerve Growth Factors Nuclear Proteins Phosphoproteins Transcription Factors Epidermal Growth Factor Zinc Tetradecanoylphorbol Acetate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cao X M
Department of Medicine, University of Chicago, Illinois 60637.
Koski R A
Gashler A
McKiernan M
Morris C F
Gaffney R
Hay R V
Sukhatme V P
References (37)
37 references, click to expand
  1. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  2. Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene.
    Neuron. 1988 May;1(3):183-8 PMID: 3272167
  3. The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
    FEBS Lett. 1985 Jul 8;186(2):271-4 PMID: 4007166
  4. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  5. Human proto-oncogene N-myc encodes nuclear proteins that bind DNA.
    Mol Cell Biol. 1986 Dec;6(12):4450-7 PMID: 3796607
  6. Recombinant-DNA-derived bovine growth hormone from Escherichia coli. 1. Demonstration that the hormone is expressed in reduced form, and isolation of the hormone in oxidized, native form.
    Eur J Biochem. 1987 Mar 2;163(2):313-21 PMID: 3545831
  7. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  8. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  9. Structure of mouse metallothionein-I gene and its mRNA.
    Nature. 1981 Jul 16;292(5820):267-9 PMID: 7254320
  10. Purification and immunological properties of proton-ATPase complexes from yeast and rat liver mitochondria.
    J Biol Chem. 1981 Sep 10;256(17):9224-8 PMID: 6455439
  11. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  12. Metallothionein-human GH fusion genes stimulate growth of mice.
    Science. 1983 Nov 18;222(4625):809-14 PMID: 6356363
  13. Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.
    J Biol Chem. 1983 Dec 10;258(23):14120-5 PMID: 6196359
  14. Viral and cellular fos proteins: a comparative analysis.
    Cell. 1984 Feb;36(2):259-68 PMID: 6319013
  15. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  16. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
    Cell. 1987 Dec 24;51(6):1079-90 PMID: 3319186
  17. Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA.
    Nature. 1988 Mar 17;332(6161):284-6 PMID: 2831463
  18. A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization.
    Cell. 1988 Apr 8;53(1):37-43 PMID: 3127059
  19. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
    EMBO J. 1988 Jan;7(1):29-35 PMID: 3129290
  20. A novel early growth response gene rapidly induced by fibroblast, epithelial cell and lymphocyte mitogens.
    Oncogene Res. 1987 Sep-Oct;1(4):343-55 PMID: 3130602
  21. fra-1: a serum-inducible, cellular immediate-early gene that encodes a fos-related antigen.
    Mol Cell Biol. 1988 May;8(5):2063-9 PMID: 3133553
  22. Cloning of tetradecanoyl phorbol ester-induced 'primary response' sequences and their expression in density-arrested Swiss 3T3 cells and a TPA non-proliferative variant.
    Oncogene. 1987;1(3):263-70 PMID: 3330774
  23. Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4691-5 PMID: 3133658
  24. Heavy metal ions in transcription factors from HeLa cells: Sp1, but not octamer transcription factor requires zinc for DNA binding and for activator function.
    Nucleic Acids Res. 1988 Jul 11;16(13):5771-81 PMID: 3135532
  25. Transcriptional activation of c-jun during the G0/G1 transition in mouse fibroblasts.
    Nature. 1988 Aug 11;334(6182):535-7 PMID: 3136397
  26. Induction of proto-oncogene JUN/AP-1 by serum and TPA.
    Nature. 1988 Aug 18;334(6183):629-31 PMID: 2457172
  27. 5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene.
    Nucleic Acids Res. 1988 Sep 26;16(18):8835-46 PMID: 3140220
  28. Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with "zinc-binding finger" structure.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7164-8 PMID: 3140236
  29. Apolipoproteins of the orotic acid fatty liver: implications for the biogenesis of plasma lipoproteins.
    J Lipid Res. 1988 Aug;29(8):981-95 PMID: 3183522
  30. The yeast regulatory protein ADR1 binds in a zinc-dependent manner to the upstream activating sequence of ADH2.
    Mol Cell Biol. 1988 Oct;8(10):4552-6 PMID: 3141794
  31. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7857-61 PMID: 3141919
  32. A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8444-8 PMID: 3186734
  33. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.
    Cell. 1988 Dec 23;55(6):989-1003 PMID: 3203386
  34. Allelotype of colorectal carcinomas.
    Science. 1989 Apr 14;244(4901):207-11 PMID: 2565047
  35. The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.
    EMBO J. 1989 Mar;8(3):805-13 PMID: 2498083
  36. DNA binding site of the growth factor-inducible protein Zif268.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8737-41 PMID: 2510170
  37. Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):711-6 PMID: 6514007
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.1931-1939.1990
Published
1990-05-00
Pages
1931-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360539
Subset
IM
Grants
NIDDK NIH HHS · DK 13914-20 · United States
NHLBI NIH HHS · HL 15062 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.5.1931-1939.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2