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PMID: 2355916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of cyr61, a growth factor-inducible immediate-early gene.

Molecular and cellular biology ·Vol. 10 ·No. 7 ·1990-07-00 ·Pages 3569-77

O'Brien TP, Yang GP, Sanders L, Lau LF

Abstract

A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle. Cyr61 contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Division Cell Line Cells, Cultured Culture Media Cysteine-Rich Protein 61 DNA/genetics,isolation & purification Gene Expression Genes/drug effects Growth Substances/genetics Humans Immediate-Early Proteins Intercellular Signaling Peptides and Proteins Mice Molecular Sequence Data Platelet-Derived Growth Factor/pharmacology Protein Biosynthesis Proteins/genetics RNA, Messenger/genetics,isolation & purification Restriction Mapping Transcription, Genetic
Chemicals
CCN1 protein, human CCN1 protein, mouse Culture Media Cysteine-Rich Protein 61 Growth Substances Immediate-Early Proteins Intercellular Signaling Peptides and Proteins Platelet-Derived Growth Factor Proteins RNA, Messenger DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Brien T P
Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
Yang G P
Sanders L
Lau L F
References (55)
55 references, click to expand
  1. A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization.
    Cell. 1988 Apr 8;53(1):37-43 PMID: 3127059
  2. A gene activated by growth factors is related to the oncogene v-jun.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 PMID: 3422745
  3. Regulation of human thymidine kinase during the cell cycle.
    J Biol Chem. 1988 Jun 15;263(17):8350-8 PMID: 3372530
  4. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3738-42 PMID: 3287374
  5. Cloning, characterization, and expression of a human insulin-like growth factor binding protein.
    Biochem Biophys Res Commun. 1988 May 16;152(3):1289-97 PMID: 2454104
  6. fra-1: a serum-inducible, cellular immediate-early gene that encodes a fos-related antigen.
    Mol Cell Biol. 1988 May;8(5):2063-9 PMID: 3133553
  7. Complexity of the early genetic response to growth factors in mouse fibroblasts.
    Mol Cell Biol. 1988 May;8(5):2140-8 PMID: 2898731
  8. Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4691-5 PMID: 3133658
  9. Transcriptional activation of c-jun during the G0/G1 transition in mouse fibroblasts.
    Nature. 1988 Aug 11;334(6182):535-7 PMID: 3136397
  10. Epidermal growth factor stimulates transcription of the c-jun proto-oncogene in rat fibroblasts.
    Nature. 1988 Aug 11;334(6182):538-9 PMID: 3136398
  11. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin.
    EMBO J. 1988 Jul;7(7):2025-33 PMID: 2970963
  12. Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with "zinc-binding finger" structure.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7164-8 PMID: 3140236
  13. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7857-61 PMID: 3141919
  14. A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8444-8 PMID: 3186734
  15. Induction of protooncogene c-jun by serum growth factors.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8464-7 PMID: 3186736
  16. Isolation and characterization of a cDNA encoding the low molecular weight insulin-like growth factor binding protein (IBP-1).
    EMBO J. 1988 Aug;7(8):2417-23 PMID: 2461294
  17. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.
    Cell. 1988 Dec 23;55(6):989-1003 PMID: 3203386
  18. Coordinate induction of fibronectin, fibronectin receptor, tropomyosin, and actin genes in serum-stimulated fibroblasts.
    Exp Cell Res. 1989 Feb;180(2):537-45 PMID: 2521606
  19. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins.
    J Biol Chem. 1989 Mar 5;264(7):4133-7 PMID: 2917992
  20. Identification of a phorbol ester-repressible v-src-inducible gene.
    Proc Natl Acad Sci U S A. 1989 Feb;86(4):1178-82 PMID: 2537491
  21. The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.
    EMBO J. 1989 Mar;8(3):805-13 PMID: 2498083
  22. Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 variants can occur through protein kinase C-dependent and -independent pathways.
    Mol Cell Biol. 1989 Apr;9(4):1790-3 PMID: 2471069
  23. Genomic response to growth factors.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:893-900 PMID: 3151190
  24. Inducible overexpression and secretion of int-1 protein.
    Mol Cell Biol. 1989 Aug;9(8):3377-84 PMID: 2677670
  25. Serum-inducible genes.
    Adv Cancer Res. 1989;53:1-32 PMID: 2678945
  26. DNA binding site of the growth factor-inducible protein Zif268.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8737-41 PMID: 2510170
  27. G1 events and regulation of cell proliferation.
    Science. 1989 Nov 3;246(4930):603-8 PMID: 2683075
  28. Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor.
    EMBO J. 1989 Nov;8(11):3327-35 PMID: 2555161
  29. The human homolog of the JE gene encodes a monocyte secretory protein.
    Mol Cell Biol. 1989 Nov;9(11):4687-95 PMID: 2513477
  30. Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene.
    Neuron. 1988 May;1(3):183-8 PMID: 3272167
  31. Nucleotide sequence and expression of a cDNA clone encoding a fetal rat binding protein for insulin-like growth factors.
    J Biol Chem. 1989 Mar 25;264(9):5148-54 PMID: 2538475
  32. Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):1850-4 PMID: 2648385
  33. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  34. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  35. Regulation of human histone gene expression: kinetics of accumulation and changes in the rate of synthesis and in the half-lives of individual histone mRNAs during the HeLa cell cycle.
    Mol Cell Biol. 1983 Apr;3(4):539-50 PMID: 6406835
  36. Molecular cloning of gene sequences regulated by platelet-derived growth factor.
    Cell. 1983 Jul;33(3):939-47 PMID: 6872001
  37. Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.
    Mol Cell Biol. 1983 Oct;3(10):1738-45 PMID: 6646120
  38. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
  39. Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells.
    Biochem Biophys Res Commun. 1984 May 16;120(3):1053-9 PMID: 6233972
  40. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  41. Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b.
    J Biol Chem. 1985 Feb 10;260(3):1513-20 PMID: 2981859
  42. Levels of c-myc oncogene mRNA are invariant throughout the cell cycle.
    Nature. 1985 Mar 28-Apr 3;314(6009):363-6 PMID: 3982504
  43. c-myc oncogene protein synthesis is independent of the cell cycle in human and avian cells.
    Nature. 1985 Mar 28-Apr 3;314(6009):366-9 PMID: 3885045
  44. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  45. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.
    EMBO J. 1985 Dec 1;4(12):3145-51 PMID: 3841511
  46. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  47. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene.
    Mol Cell Biol. 1985 Dec;5(12):3337-44 PMID: 3018519
  48. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  49. Early signals in the mitogenic response.
    Science. 1986 Oct 10;234(4773):161-6 PMID: 3018928
  50. Somatomedin (insulin-like growth factors)-binding proteins. Molecular forms and regulation.
    Horm Res. 1986;24(2-3):141-51 PMID: 2428721
  51. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6 PMID: 3469660
  52. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188-92 PMID: 2890161
  53. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  54. The int-1 proto-oncogene products are glycoproteins that appear to enter the secretory pathway.
    Mol Cell Biol. 1987 Nov;7(11):3978-84 PMID: 3323883
  55. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
    EMBO J. 1988 Jan;7(1):29-35 PMID: 3129290
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.7.3569-3577.1990
Published
1990-07-00
Pages
3569-77
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360792
Subset
IM
Grants
NCI NIH HHS · R01 CA46565 · United States
NCI NIH HHS · R01 CA52220 · United States
Databases
GENBANK
M32490
External Links
PubMed source DOI: 10.1128/mcb.10.7.3569-3577.1990 PMC Full Text View journal details
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